Related Experiment Video

Updated: Jul 25, 2025

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment
11:13

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

1.6K

Phellodendronoside A Exerts Anticancer Effects Depending on Inducing Apoptosis Through ROS/Nrf2/Notch Pathway and

Yanpo Si1,2, Chengcheng Hui1, Tao Guo1,2

  • 1Department of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, People's Republic of China.

Journal of Hepatocellular Carcinoma
|June 26, 2023
PubMed
Abstract

Insights

This study reveals that Phellodendri Cortex extract (PDA) inhibits hepatocellular carcinoma cell proliferation by targeting the ROS/Nrf2/Notch signaling pathway and altering metabolic profiles, suggesting its potential as a therapeutic agent.

Area of Science:

  • Pharmacology
  • Cell Biology
  • Biochemistry

Background:

  • Hepatocellular carcinoma (HCC) remains a significant global health challenge.
  • Identifying novel therapeutic agents for HCC is crucial.

Purpose of the Study:

  • To elucidate the mechanism by which Phellodendri Cortex extract (PDA) affects hepatocellular carcinoma SMMC-7721 cells in vitro.
  • To investigate PDA's potential as a therapeutic agent for HCC.

Main Methods:

  • Assessed cytotoxic activity, colony formation, cell cycle distribution, and apoptosis.
  • Analyzed intracellular reactive oxygen species (ROS) and Ca2+ levels.
  • Investigated protein expression in Nrf2 and Notch pathways and metabolite profiles.

Main Results:

  • PDA demonstrated dose-dependent inhibition of cell proliferation and migration, increased ROS and Ca2+ levels, and induced apoptosis.
  • PDA suppressed the activation of key proteins in the Nrf2 and Notch signaling pathways.
  • Metabonomic analysis revealed PDA significantly regulated metabolites, particularly those related to bile acid biosynthesis and carnitine derivatives, and modulated the Notch signaling pathway.

Conclusions:

  • PDA inhibits HCC cell proliferation by targeting the ROS/Nrf2/Notch signaling pathway.
  • PDA significantly alters the metabolic profile of HCC cells.
  • PDA shows promise as a potential therapeutic agent for hepatocellular carcinoma.
Keywords:
acylcarnitineapoptosisbile acidsisoquinoline alkaloidmetabolomics

More Related Videos

Establishment of Hepatocarcinoma in BALB/c-nu Mice and Investigation of the Therapeutic Effect of the Sanleng Jiashen Formula09:03

Establishment of Hepatocarcinoma in BALB/c-nu Mice and Investigation of the Therapeutic Effect of the Sanleng Jiashen Formula

Published on: January 26, 2024

411
Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen19:44

Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen

Published on: May 30, 2012

18.7K

Related Concept Videos

The Extrinsic Apoptotic Pathway01:17

The Extrinsic Apoptotic Pathway

The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
6.5K
Apoptosis01:30

Apoptosis

Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
11.6K
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
6.6K
Phagocytosis of Apoptotic Cells01:17

Phagocytosis of Apoptotic Cells

Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or  immature dendritic cells. Non-professional phagocytes such as  epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes. 
Normal cells contain receptors that prevent them from being recognized...
3.9K
Overview of Cell Death01:30

Overview of Cell Death

Cell death is an essential process where the body gets rid of old or damaged cells. Cell proliferation and death need to be balanced, as an imbalance between the two may lead to cancer or autoimmune diseases.
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
7.4K