Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Oogenesis02:07

Oogenesis

63.9K
In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
63.9K
Meiosis II02:02

Meiosis II

45.9K
Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
45.9K
S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

4.7K
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...
4.7K
Replication in Prokaryotes01:32

Replication in Prokaryotes

25.1K
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
25.1K
Replication in Eukaryotes01:29

Replication in Eukaryotes

14.0K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
14.0K
Meiosis I01:49

Meiosis I

193.8K
Meiosis is a carefully orchestrated set of cell divisions, the goal of which—in humans—is to produce haploid sperm or eggs, each containing half the number of chromosomes present in somatic cells elsewhere in the body. Meiosis I is the first such division, and involves several key steps, among them: condensation of replicated chromosomes in diploid cells; the pairing of homologous chromosomes and their exchange of information; and finally, the separation of homologous chromosomes by...
193.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Chemical Defensome: A Survey of Environmental Sensing and Response Genes in Copepods.

International journal of molecular sciences·2025
Same author

The NEREA Augmented Observatory: an integrative approach to marine coastal ecology.

Scientific data·2024
Same author

Extreme genome scrambling in marine planktonic <i>Oikopleura dioica</i> cryptic species.

Genome research·2024
Same author

De novo transcriptomes of six calanoid copepods (Crustacea): a resource for the discovery of novel genes.

Scientific data·2023
Same author

Chemosensory-Related Genes in Marine Copepods.

Marine drugs·2022
Same author

Physiological acclimatization in high-latitude zooplankton.

Molecular ecology·2022
Same journal

Correction to: Aquatic Turning Performance in Juvenile Loggerhead and Green Sea Turtles.

Integrative organismal biology (Oxford, England)·2026
Same journal

Bridging Science Across Species: A Biomechanics Outreach Event at the Zoo.

Integrative organismal biology (Oxford, England)·2026
Same journal

You Should Look a Gift Ungulate in the Mouth: Using 2D Occlusal Cheek Tooth Morphology to Study the Evolution of Molarization in Ungulates.

Integrative organismal biology (Oxford, England)·2026
Same journal

Aquifer-Mediated Speciation in Cave-Adapted Fishes.

Integrative organismal biology (Oxford, England)·2026
Same journal

Biology of Superpowers: A Curriculum Activity for Teaching Adaptation, Trade-offs, and Organismal Diversity.

Integrative organismal biology (Oxford, England)·2026
Same journal

From Fin to Limb: Orientational Shift and Evolution of Diagonal-Couplet Gait in Tetrapods.

Integrative organismal biology (Oxford, England)·2026
See all related articles

Related Experiment Video

Updated: Jul 25, 2025

In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining
10:31

In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining

Published on: October 15, 2016

9.2K

Post-Diapause DNA Replication during Oogenesis in a Capital-Breeding Copepod.

K J Monell1,2, V Roncalli3, R R Hopcroft4

  • 1Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, Michigan 48109, USA.

Integrative Organismal Biology (Oxford, England)
|June 26, 2023
PubMed
Summary
This summary is machine-generated.

In Neocalanus flemingeri, oocyte production is sequential, with DNA replication limited to the initial weeks post-diapause. This strategy separates egg formation from provisioning, ensuring high-quality offspring in resource-limited environments.

More Related Videos

Author Spotlight: Understanding DNA Damage Response in Mammalian Oocytes and Preimplantation Embryos
07:46

Author Spotlight: Understanding DNA Damage Response in Mammalian Oocytes and Preimplantation Embryos

Published on: June 23, 2023

2.5K
Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II
10:39

Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II

Published on: February 26, 2018

15.6K

Related Experiment Videos

Last Updated: Jul 25, 2025

In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining
10:31

In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining

Published on: October 15, 2016

9.2K
Author Spotlight: Understanding DNA Damage Response in Mammalian Oocytes and Preimplantation Embryos
07:46

Author Spotlight: Understanding DNA Damage Response in Mammalian Oocytes and Preimplantation Embryos

Published on: June 23, 2023

2.5K
Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II
10:39

Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II

Published on: February 26, 2018

15.6K

Area of Science:

  • Marine Biology
  • Reproductive Biology
  • Arthropod Physiology

Background:

  • High-latitude arthropods enter diapause (dormancy) to survive harsh conditions and optimize reproduction.
  • Neocalanus flemingeri, a subarctic copepod, has decoupled feeding from oogenesis, necessitating resource regulation for egg production.
  • The mechanism by which N. flemingeri limits oocyte formation remains uninvestigated.

Purpose of the Study:

  • To investigate the timing and regulation of oocyte production in post-diapause Neocalanus flemingeri females.
  • To determine if and how N. flemingeri limits oocyte formation to ensure egg quality.

Main Methods:

  • Females were incubated with 5-Ethynyl-2'-deoxyuridine (EdU) to label cells undergoing DNA replication.
  • Ovary and oviduct DNA replication was assessed post-diapause termination.
  • EdU incorporation was quantified over time to determine the phase of oocyte production.

Main Results:

  • Both oogonia and oocytes incorporated EdU, indicating DNA replication during oogenesis.
  • EdU labeling peaked at 72 hours post-diapause and remained high for two weeks.
  • No labeling was detected by four weeks post-diapause, preceding spawning.

Conclusions:

  • Oogenesis in N. flemingeri is sequential, with oocyte formation initiated within 24 hours of diapause termination and limited to the first few weeks.
  • This sequential strategy separates oocyte production from provisioning, ensuring fully provisioned eggs.
  • This contrasts with the concurrent oocyte maturation seen in most copepods, suggesting a unique reproductive strategy.