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Related Concept Videos

Clinical Applications of Epidermal Stem Cells01:19

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Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...
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Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding
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A versatile, bioengineered skin reconstruction device designed for use in austere environments.

Joachim G S Veit1,2, Morgan Weidow1, Monica A Serban1,2

  • 1Serban Lab, Department of Biomedical and Pharmaceutical Sciences, University of Montana, Missoula, MT, United States.

Frontiers in Bioengineering and Biotechnology
|June 26, 2023
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Summary

A novel silk fibroin and hyaluronic acid skin substitute was developed for austere environments. This biocompatible device promotes healing, delivers drugs topically, and adheres without trained personnel.

Keywords:
austere environmentbiomaterialhyaluronic acidmicroneedlessilk fibroinskin reconstruction devicewound healing

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Medical Device Design

Background:

  • Existing skin substitutes are inadequate for severe injuries in resource-limited settings.
  • Austere environments present unique challenges for medical device design and deployment.
  • Ideal devices for such settings require biocompatibility, bioresorbability, and ease of use.

Purpose of the Study:

  • To develop and validate a novel skin substitute for treating severe wounds in austere environments.
  • To create a device that is biocompatible, promotes tissue healing, and enables topical drug delivery.
  • To design a self-adherent device deployable without trained medical personnel.

Main Methods:

  • Formulation of a silk fibroin and hyaluronic acid derivative composite.
  • Mechanical testing, including ultimate tensile strength and adhesive strength evaluation.
  • In vitro assessment of cytocompatibility, reactive oxygen species (ROS) protection, and skin irritation.
  • Evaluation of topical drug delivery enhancement using a microneedle array.
  • In vitro full-thickness skin wound model for assessing wound healing potential.

Main Results:

  • The device exhibited a tensile strength of 438.0 KPa and adhesive strength of 12.0 MPa.
  • Hyaluronic acid derivative pre-treatment rescued dermal fibroblast viability from 49.7% to 85.0% against ROS.
  • A 223% increase in small molecule epidermal permeation was observed with the integrated microneedle array.
  • The device demonstrated no skin irritation in vitro and showed promising integration and cellular migration in a wound model.

Conclusions:

  • The developed silk fibroin and hyaluronic acid prototype is mechanically robust and cytocompatible.
  • The device offers protection from ROS, enhances topical drug delivery, and is self-adherent.
  • This innovative skin substitute shows significant potential for wound healing in austere environments.