Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Induced-fit Model01:13

Induced-fit Model

81.2K
Most chemical reactions in cells require enzymes—biological catalysts that speed up the reaction without being consumed or permanently changed. They reduce the activation energy needed to convert the reactants into products. Enzymes are proteins, that usually work by binding to a substrate—a reactant molecule that they act upon.
Enzymes exhibit substrate specificity, meaning that they can only bind to certain substrates. This is mainly determined by the shape and chemical...
81.2K
The Equilibrium Binding Constant and Binding Strength02:18

The Equilibrium Binding Constant and Binding Strength

13.0K
The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:
13.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A computational pipeline for predicting distal hotspots in an artificial enzyme.

International journal of biological macromolecules·2025
Same author

Radical Formation by Direct Single Electron Transfer between Nitrobenzene and Anionic Organo Bases.

ACS omega·2025
Same author

<i>In Silico</i> Assessment of Biomolecule Reactivity with Leachables.

PDA journal of pharmaceutical science and technology·2024
Same author

Distal mutations enhance efficiency of free and immobilized NOV1 dioxygenase for vanillin synthesis.

Journal of biotechnology·2024
Same author

Computation-guided engineering of distal mutations in an artificial enzyme.

Faraday discussions·2024
Same author

Correction: Analysis of Poly(ethylene terephthalate) degradation kinetics of evolved IsPETase variants using a surface crowding mode.

The Journal of biological chemistry·2024

Related Experiment Video

Updated: Jul 25, 2025

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
11:13

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

Published on: August 20, 2018

11.1K

Enzyme immobilization studied through molecular dynamic simulations.

Nicholus Bhattacharjee1, Lur Alonso-Cotchico1, Maria Fátima Lucas1

  • 1Zymvol Biomodeling SL, Barcelona, Spain.

Frontiers in Bioengineering and Biotechnology
|June 26, 2023
PubMed
Summary

Molecular dynamic simulations offer insights into enzyme immobilization strategies. While simulations explain molecular aspects, predicting protocols to control enzyme activity and stability remains a challenge.

Keywords:
carbon nanotubeenzyme immobilizationgraphenemolecular dynamics simulationsnanoparticlesself assembled monolayers

More Related Videos

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry
11:20

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry

Published on: March 29, 2018

7.6K
Study of Protein Dynamics via Neutron Spin Echo Spectroscopy
08:03

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy

Published on: April 13, 2022

2.1K

Related Experiment Videos

Last Updated: Jul 25, 2025

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
11:13

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

Published on: August 20, 2018

11.1K
Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry
11:20

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry

Published on: March 29, 2018

7.6K
Study of Protein Dynamics via Neutron Spin Echo Spectroscopy
08:03

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy

Published on: April 13, 2022

2.1K

Area of Science:

  • Biotechnology and Biochemistry
  • Computational Chemistry
  • Enzyme Engineering

Background:

  • Experimental methods have limitations in elucidating molecular-level impacts of enzyme immobilization.
  • Enzyme immobilization is crucial for enhancing enzyme stability and activity in various applications.
  • Molecular simulations provide a powerful tool to study enzyme-surface interactions.

Purpose of the Study:

  • To review the application of molecular dynamic simulations in understanding enzyme immobilization.
  • To analyze the impact of different immobilization surfaces on enzyme properties.
  • To identify the current limitations and future directions in simulation-based enzyme immobilization research.

Main Methods:

  • Literature review of computational studies on enzyme immobilization.
  • Focus on molecular dynamic simulations applied to various surfaces.
  • Analysis of simulation data to understand enzyme-surface interactions and their effects.

Main Results:

  • Simulations effectively characterize surface phenomena during enzyme immobilization.
  • Computational studies cover immobilization on nanoparticles, self-assembled monolayers, graphene, and carbon nanotubes.
  • Simulations primarily rationalize molecular aspects rather than predict control protocols.

Conclusions:

  • Molecular dynamic simulations are valuable for understanding enzyme immobilization mechanisms.
  • There is a gap in using simulations to predict and control immobilization protocols for optimal enzyme performance.
  • Future research should focus on developing predictive simulation models for enzyme immobilization.