Related Experiment Video
Updated: Jul 25, 2025

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
Published on: August 15, 2014
Millimetric scale two-photon Bessel-Gauss beam light sheet microscopy with three-axis isotropic resolution using an
Cléophace Akitegetse1,2,3, Thomas Charland1,2, Mireille Quémener1,2,4
1Centre de recherche CERVO, Québec, Québec, Canada.
Significance:
Typical light sheet microscopes suffer from artifacts related to the geometry of the light sheet. One main inconvenience is the non-uniform thickness of the light sheet obtained with a Gaussian laser beam.
Aim:
We developed a two-photon light sheet microscope that takes advantage of a thin and long Bessel-Gauss beam illumination to increase the sheet extent without compromising the resolution.
Approach:
We use an axicon lens placed directly at the output of an amplified femtosecond laser to produce a long Bessel-Gauss beam on the sample. We studied the dopaminergic system and its projections in a whole cleared mouse brain.
Results:
Our light sheet microscope allows an isotropic resolution of in all three axes of the scanned volume while keeping a millimetric-sized field of view, and a fast acquisition rate of up to . With slight modifications to the optical setup, the sheet extent can be increased to 6 mm.
Conclusion:
The proposed system's sheet extent and resolution surpass currently available systems, enabling the fast imaging of large specimens.
More Related Videos
12:44Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM
Published on: September 29, 2014
08:32Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
Published on: January 26, 2024
Related Concept Videos
Three-Dimensional Microscopy in Microbiology
Confocal Fluorescence Microscopy
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Super-resolution Fluorescence Microscopy