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Aminosilane Functionalized Aligned Fiber PCL Scaffolds for Peripheral Nerve Repair.

Caroline S Taylor1, Joseph Barnes2, Manohar Prasad Koduri2

  • 1Department of Materials Science & Engineering, Kroto Research Institute, Broad Lane, Sheffield, S3 7HQ, UK.

Macromolecular Bioscience
|June 26, 2023
PubMed
Summary

Aminosilane modification of Polycaprolactone (PCL) scaffolds enhances nerve regeneration. This cost-effective method supports neuronal and Schwann cell viability and promotes neurite outgrowth for improved nerve guidance devices.

Keywords:
aminosilaneselectrospun fibersnerve regenerationperipheral nervessilane modificationtopographical guidance

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Neuroscience

Background:

  • Silane modification offers a cost-effective method to functionalize biomaterials for tissue engineering.
  • Aminosilane deposition controls cell adhesion and differentiation by introducing amine groups.
  • Peripheral nerve regeneration requires advanced biomaterials for guidance and support.

Purpose of the Study:

  • To investigate the efficacy of 11-aminoundecyltriethoxysilane (CL11) modification on aligned Polycaprolactone (PCL) scaffolds for peripheral nerve regeneration.
  • To evaluate the impact of CL11-modified PCL scaffolds on neuronal and Schwann cell behavior in vitro and ex vivo.
  • To establish a reproducible method for enhancing nerve guidance devices.

Main Methods:

  • Aligned PCL scaffolds were fabricated using electrospinning.
  • Scaffolds were modified with CL11 aminosilane.
  • Surface characterization included water contact angle, AFM, and XPS.
  • In vitro studies involved NG108-15 neuronal cells and primary Schwann cells.
  • Ex vivo studies utilized Dorsal Root Ganglion (DRG) explants.

Main Results:

  • CL11 modification was confirmed on PCL fibers.
  • CL11-modified PCL scaffolds significantly enhanced NG108-15 neuronal cell and Schwann cell viability.
  • Neuronal cell differentiation and Schwann cell phenotype were maintained on modified scaffolds.
  • Ex vivo cultures showed increased Schwann cell migration and neurite outgrowth on CL11-PCL scaffolds.
  • CL11-modified scaffolds outperformed unmodified scaffolds in supporting nerve regeneration.

Conclusions:

  • Aminosilane modification of PCL scaffolds is a viable and cost-effective strategy for peripheral nerve regeneration.
  • CL11-modified PCL scaffolds provide a conducive environment for neuronal and Schwann cell functions.
  • This approach offers a promising enhancement for nerve guidance devices to improve nerve regeneration outcomes.