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Related Experiment Video

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Surface-enhanced fluorescence for lipopolysaccharide analysis based on shell-isolated nanoparticle.

Weidao Yu1, Wen Sun1, Ketong Guo1

  • 1College of Food and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, PR China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|June 26, 2023
PubMed
Summary

A novel surface-enhanced fluorescent sensor using shell-isolated nanoparticles was developed for sensitive lipopolysaccharide (LPS) detection. This advancement offers potential for rapid LPS analysis in food and biomedical applications.

Keywords:
CdTe quantum dotsLipopolysaccharideShell-isolated nanoparticlesSurface-enhanced fluorescence

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Area of Science:

  • Nanotechnology
  • Biomedical Engineering
  • Analytical Chemistry

Background:

  • Lipopolysaccharide (LPS) from gram-negative bacteria is a potent inflammatory inducer, triggering multi-system host responses.
  • Accurate and rapid detection of LPS is crucial for biomedical diagnosis and food safety.
  • Existing detection methods may lack sensitivity or require complex procedures.

Purpose of the Study:

  • To develop a highly sensitive surface-enhanced fluorescent (SEF) sensor for lipopolysaccharide (LPS) detection.
  • To utilize shell-isolated nanoparticles (SHINs) for signal amplification in LPS analysis.
  • To validate the sensor's performance in complex biological and food matrices.

Main Methods:

  • Fabrication of a SEF sensor using silica shell-coated gold nanoparticles (Au NPs) to enhance the fluorescence of CdTe quantum dots (QDs).
  • Utilized 3D finite-difference time-domain (3D-FDTD) simulations to understand the electric field amplification mechanism.
  • Assessed the sensor's linear detection range (0.1-20 μg/mL) and limit of detection (64 ng/mL).

Main Results:

  • Demonstrated significant fluorescent signal enhancement of CdTe QDs due to local electric field amplification by Au NPs.
  • Achieved a low limit of detection (64 ng/mL) for LPS.
  • Successfully applied the sensor for LPS detection in milk and human serum samples with high selectivity.

Conclusions:

  • The developed SEF sensor based on SHINs provides a sensitive and selective platform for LPS detection.
  • This technology holds significant potential for improving early diagnosis in biomedical settings and ensuring food safety.
  • Further research can explore its application in detecting other bacterial components or biomarkers.