Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

10.7K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
10.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PIKfyve influences inter-organelle contacts with lysosomes to modulate the endoplasmic reticulum.

The Journal of cell biology·2026
Same author

Effect of ultrasound-microbubble exposure on acute myeloid leukemia cancer cell proteome.

Scientific reports·2025
Same author

Depletion of endomembrane reservoirs drives phagocytic appetite exhaustion in macrophages.

Journal of cell science·2025
Same author

Photoinduced luminescence activation of hydrophilic 'caged' carbons dots.

Nanoscale·2025
Same author

INPP4B promotes PDAC aggressiveness via PIKfyve and TRPML-1-mediated lysosomal exocytosis.

The Journal of cell biology·2024
Same author

The LCLAT1/LYCAT acyltransferase is required for EGF-mediated phosphatidylinositol-3,4,5-trisphosphate generation and Akt signaling.

Molecular biology of the cell·2024

Related Experiment Video

Updated: Jul 25, 2025

High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization
09:22

High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization

Published on: November 27, 2014

14.8K

Quantifying Phagocytosis by Immunofluorescence and Microscopy.

Sierra Soffiaturo1,2, Christopher Choy1,2,3, Roberto J Botelho4,5

  • 1Molecular Science Graduate Program, Toronto Metropolitan University, Toronto, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|June 26, 2023
PubMed
Summary
This summary is machine-generated.

This study introduces a fluorescence microscopy method to quantify phagocytosis by immune cells like macrophages. The technique measures the engulfment of particles such as bacteria, aiding infection resolution and tissue health.

Keywords:
ImmunofluorescenceMicroscopyPhagocytic indexPhagocytosisPhagosomeQuantification

More Related Videos

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy
06:15

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

Published on: August 18, 2018

12.7K
Measuring Phagosome pH by Ratiometric Fluorescence Microscopy
14:39

Measuring Phagosome pH by Ratiometric Fluorescence Microscopy

Published on: December 7, 2015

14.0K

Related Experiment Videos

Last Updated: Jul 25, 2025

High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization
09:22

High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization

Published on: November 27, 2014

14.8K
Quantification of Efferocytosis by Single-cell Fluorescence Microscopy
06:15

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

Published on: August 18, 2018

12.7K
Measuring Phagosome pH by Ratiometric Fluorescence Microscopy
14:39

Measuring Phagosome pH by Ratiometric Fluorescence Microscopy

Published on: December 7, 2015

14.0K

Area of Science:

  • Immunology
  • Cell Biology
  • Microscopy

Background:

  • Phagocytosis is a critical immune process where cells like macrophages engulf pathogens and cellular debris.
  • This process is vital for clearing infections and maintaining tissue homeostasis.
  • Phagocytosis involves complex signaling pathways regulating actin dynamics and membrane remodeling.

Purpose of the Study:

  • To develop and present a fluorescence microscopy-based technique for quantifying phagocytosis.
  • To provide a method for assessing the efficiency of phagocytic cells.

Main Methods:

  • Utilized a macrophage-like cell line for experiments.
  • Employed fluorescence microscopy to visualize and quantify particle uptake.
  • Tested the method using antibody-opsonized polystyrene beads and Escherichia coli.

Main Results:

  • Successfully demonstrated a quantifiable method for measuring phagocytosis rates.
  • Validated the technique with common phagocytic targets.

Conclusions:

  • The presented fluorescence microscopy technique offers a robust way to quantify phagocytosis.
  • This method is adaptable for various phagocytes and particulate materials, advancing research in immunology and cell biology.