Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

MERTK coordinates efferocytosis by regulating integrin localization and activation.

Journal of cell science·2026

Same author

GATA2 Mediates Macrophage Proliferation During Atherosclerosis.

Journal of the American Heart Association·2025

Same author

B-cell subsets have different capacities for phagocytosis and subsequent presentation of antigen to cognate T cells.

Journal of immunology (Baltimore, Md. : 1950)·2025

Same author

GATA2 induces a stem cell-like transcriptional program in macrophages that promotes an atherogenic phenotype.

Journal of leukocyte biology·2025

Same author

Therapeutic targeting of TGF-β in lung cancer.

The FEBS journal·2024

Same author

SARS-CoV-2 NSP5 antagonizes MHC II expression by subverting histone deacetylase 2.

Journal of cell science·2024

Search research articles

Related Experiment Video

Updated: Jul 25, 2025

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

Published on: August 18, 2018

Methods for Quantitative Efferocytosis Assays.

Nima Taefehshokr¹, Bryan Heit^2,3

¹Department of Microbiology and Immunology, and The Western Infection, Immunity and Inflammation Centre, The University of Western Ontario, London, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)

|June 26, 2023

Summary

This study presents microscopy methods to track efferocytosis, the process of clearing apoptotic cells. These techniques visualize how signaling proteins are recruited during this crucial cellular cleanup.

Keywords:

Efferocytosis Immunostaining Macrophage Method Microscopy Phagocytosis

More Related Videos

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Published on: February 14, 2022

Setting a Successful Sorting for Extracellular Vesicle Isolation

Setting a Successful Sorting for Extracellular Vesicle Isolation

Published on: October 11, 2024

Related Experiment Videos

Last Updated: Jul 25, 2025

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

Published on: August 18, 2018

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Published on: February 14, 2022

Setting a Successful Sorting for Extracellular Vesicle Isolation

Setting a Successful Sorting for Extracellular Vesicle Isolation

Published on: October 11, 2024

Area of Science:

Cell Biology
Immunology
Molecular Biology

Background:

Efferocytosis is the critical process by which apoptotic cells are cleared by phagocytes.
This process involves complex molecular signaling and protein recruitment for efficient uptake and degradation.
Understanding efferocytosis is vital for tissue homeostasis and immune regulation.

Purpose of the Study:

To develop and describe novel microscopy-based methods for quantifying efferocytic events.
To characterize the dynamic, spatiotemporal recruitment of signaling molecules during efferocytosis.
To provide versatile techniques applicable to various efferocytic cell types.

Main Methods:

Utilized genetically encoded fluorescent probes to visualize cellular processes in real-time.
Employed immunofluorescent labeling to identify and localize specific signaling proteins.
Applied microscopy techniques for enumeration of efferocytic events and analysis of protein dynamics.

Main Results:

Successfully enumerated efferocytic events using the developed microscopy methods.
Visualized and characterized the dynamic recruitment patterns of key signaling molecules during efferocytosis.
Demonstrated the applicability of these methods in macrophage models.

Conclusions:

The described microscopy methods offer a robust approach to study efferocytosis dynamics.
These techniques facilitate detailed investigation into the spatiotemporal regulation of efferocytic signaling.
The methods are adaptable for studying efferocytosis in diverse cellular contexts.