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Updated: Jul 25, 2025

Investigating the Phagocytosis of Leishmania using Confocal Microscopy
Published on: July 29, 2021
Filamentous Bacteria as Targets to Study Phagocytosis
Akriti Prashar1, Maria Cecilia Gimenez2, Serene Moussaoui2,3
1Program in Cell Biology, Peter Gilgan Centre of Research and Learning, The Hospital for Sick Children, Toronto, ON, Canada.
Abstract:
Filamentous targets are internalized via phagocytic cups that last for several minutes before closing to form a phagosome. This characteristic offers the possibility to study key events in phagocytosis with greater spatial and temporal resolution than is possible to achieve using spherical particles, for which the transition from a phagocytic cup to an enclosed phagosome occurs within a few seconds after particle attachment. In this chapter, we provide methodologies to prepare filamentous bacteria and describe how they can be used as targets to study different aspects of phagocytosis.
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