Related Experiment Video
Updated: Aug 10, 2026

Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry
Published on: July 17, 2017
The Derivation and Use of HoxB8-Driven Conditionally Immortalized Macrophages
Shranjit S Lail1, Neil McKenna2, Robin M Yates3,4,5
1Department of Medical Science, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada.
Abstract:
The use of Hox-driven conditionally immortalized immune cells has significantly increased in biomedical research over the past 15 years. HoxB8-driven conditionally immortalized myeloid progenitor cells maintain their ability to differentiate into functional macrophages. There are multiple benefits to this conditional immortalization strategy including the ability for unlimited propagation, genetic mutability, primary-like immune cells (macrophages, dendritic cells, and granulocytes) on demand, derivation from variety of mouse strains, and simple cryopreservation and reconstitution. In this chapter, we will discuss how to derive and use these HoxB8-conditionally immortalized myeloid progenitor cells.
Related Concept Videos
Combinatorial Gene Control
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Hedgehog Signaling Pathway
Multipotency of Hematopoietic Stem Cells
Zygotic Development And Stem Cell Formation
Cellular Differentiation
A zygote is a...

