Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Towards Safer Antimicrobial Peptide Therapeutics: A Predictive-Generative Framework Targeting ESKAPE Pathogens.

Probiotics and antimicrobial proteins·2026

Same author

Gene expression analysis of diabetic foot ulcers reveals the potential impact of Levofloxacin on wound healing.

Scientific reports·2025

Same author

Development of RT-LAMP assay for variants of HCV in Pakistan.

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]·2025

Same author

Amalgamation of experimental strategies, computational simulation, and computer-assisted-theoretical analysis to decipher the interaction of newly synthesized plumbagin-indole-3-propionic ester with cholinesterases.

Journal of biomolecular structure & dynamics·2025

Same author

Green Synthesis of Selenium Nanoparticles Utilizing Drimia indica: Insights Into Anticancer and Antimicrobial Activities.

Microscopy research and technique·2024

Same author

Bioremediation petroleum wastewater and oil-polluted soils by the non-toxigenic indigenous fungi.

World journal of microbiology & biotechnology·2024

Search research articles

Related Experiment Video

Updated: Jul 25, 2025

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Prospects of Microfluidic Technology in Nucleic Acid Detection Approaches.

Zilwa Mumtaz¹, Zubia Rashid², Ashaq Ali³

¹KAM School of Life Sciences, Forman Christian College University, Ferozpur Road, Lahore 54600, Pakistan.

|June 27, 2023

Summary

Microfluidic technology enables sensitive nucleic acid detection for diseases. Paper-based devices with isothermal amplification offer cost-effective, rapid point-of-care diagnostics.

Keywords:

isothermal amplification lab on chip microfluidics molecular diagnostics nucleic acid detection nucleic acid testing portable devices

More Related Videos

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

Related Experiment Videos

Last Updated: Jul 25, 2025

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

Area of Science:

Biomedical Engineering
Analytical Chemistry
Molecular Diagnostics

Background:

Conventional diagnostics require multi-step procedures, hindering rapid point-of-care (POC) testing.
Microfluidic platforms offer integrated, single-step solutions for analyte detection in clinical and biochemical applications.
Challenges in nucleic acid detection include cellular lysis, isolation, and amplification, necessitating improved on-chip methodologies.

Approach:

This review highlights microfluidic technology for nucleic acid detection of infectious and non-infectious diseases.
Explores modular microfluidics for streamlined on-chip sample preparation, amplification, and detection.
Discusses the integration of isothermal amplification with lateral flow assays for enhanced sensitivity and nanoparticle-biomolecule binding efficiency.

Key Points:

Microfluidic systems provide specific, sensitive disease detection with benefits like low cost and simple fabrication.
Paper-based microfluidics using cellulose reduce overall diagnostic costs.
CRISPR/Cas technology integration in microfluidics advances next-generation diagnostic methods.

Conclusions:

Microfluidic technology is crucial for advancing nucleic acid testing for various diseases.
Combining microfluidics with isothermal amplification and paper-based materials enhances detection limits and sensitivity.
Future prospects involve comparing diverse microfluidic systems, detection methods, and plasma separation techniques for improved diagnostics.