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Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level
Published on: June 23, 2022
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OMICS and Other Advanced Technologies in Mycological Applications
Nalin N Wijayawardene1,2,3, Nattawut Boonyuen4, Chathuranga B Ranaweera5
1Centre for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing 655011, China.
Journal of Fungi (Basel, Switzerland)
|June 27, 2023
Summary
Advanced molecular techniques like DNA sequencing and omics are revolutionizing fungal identification, especially for challenging "dark taxa". This expands our understanding of fungi
Area of Science:
- Mycology and Molecular Biology
- Ecosystem Dynamics and Fungal Roles
Background:
- Fungi are crucial to ecosystems, but precise identification is vital for various applications.
- Traditional morphological identification is limited, especially for fungi lacking distinct features ('dark taxa').
- Technological advancements offer improved fungal identification and classification.
Purpose of the Study:
- To discuss diverse taxonomic approaches for fungal identification.
- To highlight the significance of omics techniques in fungal research.
- To explore the applications of fungal knowledge in food security and medicine.
Main Methods:
- Polymerase chain reaction (PCR) amplification and DNA sequencing of ribosomal DNA (rDNA).
- Multi-loci phylogenetic analyses for robust classification.
- Application of various omics techniques: proteomics, transcriptomics, metabolomics, etc.
Main Results:
- Advanced sequencing and metagenomics aid in identifying novel fungal lineages, including 'dark taxa'.
- Omics technologies provide a comprehensive understanding of fungal biology and functions.
- These methods are critical for expanding knowledge on fungal impacts and applications.
Conclusions:
- Modern molecular and omics approaches are essential for accurate fungal taxonomy and discovery.
- Understanding fungi is key to advancements in food safety, medicine, and biotechnology.
- Further exploration of fungi in extreme and understudied environments is crucial for identifying new lineages.
Keywords:
dark taxafungal metabolitesfungal taxonomyhigh-throughput sequencingmultiomics approachesphylogenetic analysisMore Related Videos
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