Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Menopause01:28

Menopause

208
Menopause, a natural biological process marking the end of a woman's fertility, typically occurs between the fifth and sixth decade of life. This phase is characterized by the exhaustion of the ovarian follicle pool, leading to less responsive ovaries despite the high levels of Follicle Stimulating Hormone (FSH) and Luteinizing Hormone (LH). The consequential decrease in estrogen production results in symptoms like hot flashes, heavy sweating, headaches, hair loss, muscle pains, vaginal...
208
Bone Disorders01:29

Bone Disorders

3.7K
Aging and its effect on bone remodeling is the most common cause of bone disorders. In young and healthy people, bone deposition and resorption happen at an equal rate to maintain optimal bone health.
Bone deposition is also affected by the levels of sex hormones like estrogen and testosterone that promote osteoblast activity and bone matrix synthesis. When the level of these hormones decreases due to aging, it causes a reduction in bone deposition. As a result, bone resorption by osteoclasts...
3.7K
Nursing Assessment of the Genitourinary System I: Health History01:21

Nursing Assessment of the Genitourinary System I: Health History

32
The genitourinary system is critical to maintaining fluid balance, waste elimination, and reproductive function. Nurses play a vital role in assessing this system, beginning with a thorough health history. This process involves gathering patient information, identifying risk factors, and recognizing symptoms of genitourinary disorders. Early detection is vital for timely interventions and management.1. Gathering Patient InformationA complete health history includes the patient’s personal,...
32
The Effect of Aging on Tissues01:19

The Effect of Aging on Tissues

2.2K
Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...
2.2K
Urinary Bladder01:23

Urinary Bladder

1.0K
The urinary bladder is a hollow, muscular sac that temporarily stores urine before it is expelled from the body. It can hold approximately 600 mL of urine prior to micturition. The bladder is retroperitoneal and located behind the pubic symphysis in the pelvic floor.
In males, the bladder is situated in front of the rectum, while in females, it is positioned anterior to the vagina and uterus. The bladder floor contains an inverted triangular area called the trigone, defined by the two ureteric...
1.0K
Physiology of Urine Formation01:24

Physiology of Urine Formation

4.9K
Urine formation is an essential function of the human body. It plays a critical role in maintaining homeostasis by regulating the volume and composition of body fluids. The kidneys, the primary organs involved in this process, filter blood to remove waste products and excess substances, ultimately producing urine.
Glomerular Filtration
The first stage in urine formation is glomerular filtration. Each kidney contains approximately 1 million nephrons, the functional units of filtration, with a...
4.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advancing Personalized Care for Neurogenic Bladder What Medical Management Should be First Line for Bladder Storage Symptoms in NLUTD: Pro Anticholinergic.

Neurourology and urodynamics·2025
Same author

Neurotrophins and Proneurotrophins as Biomarkers for Overactive Bladder Syndrome in Aging Females.

Metabolites·2025
Same author

Bladder p75<sup>NTR</sup>-Mediated Anti-Inflammatory Response via the TLR4/TRAF6/NF-κB Axis.

Life (Basel, Switzerland)·2025
Same author

p75<sup>NTR</sup> antagonist THX-B increases mature nerve growth factor secretion by bladder cells through decreased activity of matrix metalloproteinase-9.

Molecular and cellular endocrinology·2025
Same author

2024 Canadian Urological Association guideline: Female stress urinary incontinence.

Canadian Urological Association journal = Journal de l'Association des urologues du Canada·2024
Same author

Surgery for female urethral stricture.

Neurourology and urodynamics·2024

Related Experiment Video

Updated: Jul 25, 2025

Detrusor Underactivity Model in Rats by Conus Medullaris Transection
03:26

Detrusor Underactivity Model in Rats by Conus Medullaris Transection

Published on: August 28, 2020

2.1K

Decrease in the Ratio proBDNF/BDNF in the Urine of Aging Female Patients with OAB.

Claudia Covarrubias1, Philippe G Cammisotto1, Samer Shamout1

  • 1Lady Davis Institute, McGill University, Montreal, QC H3A 0G4, Canada.

Metabolites
|June 27, 2023
PubMed
Summary
This summary is machine-generated.

The proBDNF/BDNF ratio in urine may help diagnose overactive bladder (OAB) in older women. This ratio is lower in OAB patients, potentially due to increased MMP-9 activity.

Keywords:
BDNFMMP-9microRNAsoveractive bladderproBDNFproteasesurine

More Related Videos

Ultrasonography of the Adult Male Urinary Tract for Urinary Functional Testing
05:25

Ultrasonography of the Adult Male Urinary Tract for Urinary Functional Testing

Published on: August 14, 2019

19.3K
Real-Time Void Spot Assay
06:39

Real-Time Void Spot Assay

Published on: February 10, 2023

2.0K

Related Experiment Videos

Last Updated: Jul 25, 2025

Detrusor Underactivity Model in Rats by Conus Medullaris Transection
03:26

Detrusor Underactivity Model in Rats by Conus Medullaris Transection

Published on: August 28, 2020

2.1K
Ultrasonography of the Adult Male Urinary Tract for Urinary Functional Testing
05:25

Ultrasonography of the Adult Male Urinary Tract for Urinary Functional Testing

Published on: August 14, 2019

19.3K
Real-Time Void Spot Assay
06:39

Real-Time Void Spot Assay

Published on: February 10, 2023

2.0K

Area of Science:

  • Neuroscience
  • Urology
  • Biochemistry

Background:

  • Neurotrophins like BDNF are vital for neuronal health and often imbalanced in disease.
  • Overactive bladder (OAB) is a common condition affecting aging populations.

Purpose of the Study:

  • To investigate the urinary levels of brain-derived neurotrophic factor (BDNF) and its precursor (proBDNF) in aging women with OAB.
  • To explore the diagnostic potential of the proBDNF/BDNF ratio and its relationship with OAB severity and underlying mechanisms.

Main Methods:

  • Urine samples from aging female OAB patients and healthy controls were analyzed for proBDNF and BDNF concentrations.
  • Creatinine normalization, proBDNF/BDNF ratio calculation, ROC curve analysis, and correlation with symptom scores (OABSS, IIQ-7) were performed.
  • Urinary matrix metalloproteinase-9 (MMP-9) activity and miR-491-5p levels were assessed.

Main Results:

  • While total BDNF and proBDNF levels were similar, the proBDNF/BDNF ratio was significantly lower in OAB patients (AUC = 0.729).
  • A decreased proBDNF/BDNF ratio correlated with increased OAB symptom severity.
  • Urinary MMP-9 activity was elevated in OAB, while miR-491-5p levels were decreased, suggesting enhanced proBDNF cleavage.

Conclusions:

  • The urinary proBDNF/BDNF ratio shows promise as a biomarker for OAB phenotyping in aging women.
  • Elevated MMP-9 activity, rather than translational control, appears to drive the altered proBDNF/BDNF ratio in OAB.