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Related Concept Videos

Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

446
Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which...
446
Atomic Absorption Spectroscopy: Lab01:21

Atomic Absorption Spectroscopy: Lab

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For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
 Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing...
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Atomic Absorption Spectroscopy: Instrumentation01:22

Atomic Absorption Spectroscopy: Instrumentation

805
An atomic absorption spectrophotometer (AAS) comprises several components: a radiation source, an atomizer, a monochromator, and a detector. The radiation source can be a hollow-cathode lamp (HCL) or an electrodeless-discharge lamp (EDL), both of which provide a narrow emission line of the required wavelength. However, some instruments use continuum sources and high-resolution monochromators to achieve a narrow range of radiation.
The atomizer used in AAS can be either a flame atomizer or an...
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Related Experiment Video

Updated: Jul 25, 2025

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.
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Handheld Fluorescence Spectrometer Enabling Sensitive Aflatoxin Detection in Maize.

Lien Smeesters1, Thomas Kuntzel2, Hugo Thienpont1

  • 1Department of Applied Physics and Photonics, Brussels Photonics (B-PHOT), Vrije Universiteit Brussel and Flanders Make, Pleinlaan 2, 1050 Brussels, Belgium.

Toxins
|June 27, 2023
PubMed
Summary

A novel handheld fluorescence sensor offers a non-destructive method for detecting aflatoxins in food. This technology enhances food safety by enabling rapid, on-site analysis of these harmful carcinogens.

Keywords:
aflatoxinfluorescencefood safetyhandheld spectrometermaizeoptical sensingspectroscopy

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Area of Science:

  • Food Science
  • Analytical Chemistry
  • Spectroscopy

Background:

  • Aflatoxins are significant carcinogens that pose risks to food and feed safety.
  • Current detection methods for aflatoxins are destructive and not suitable for real-time, localized analysis.
  • There is a need for rapid, non-destructive techniques for aflatoxin detection in the agrifood industry.

Purpose of the Study:

  • To develop a non-destructive optical sensing technique for aflatoxin detection using fluorescence spectroscopy.
  • To create a compact, handheld fluorescence sensing unit for on-site analysis.
  • To evaluate the sensitivity and applicability of the developed sensing unit for food safety.

Main Methods:

  • Development of a compact fluorescence sensing unit with integrated ultraviolet excitation and fluorescence detection.
  • Benchmarking the novel sensing unit against a research-grade fluorescence setup.
  • Testing the sensor's ability to detect and classify aflatoxin concentrations in maize samples.

Main Results:

  • The sensing unit demonstrated high sensitivity, spectrally separating maize powder samples with low aflatoxin concentrations (6.6 µg/kg and 11.6 µg/kg).
  • Naturally contaminated maize kernels were successfully classified across a wide range of aflatoxin levels (0 µg/kg, 0.6 µg/kg, and 1647.8 µg/kg).
  • The developed method showed good sensitivity and potential for practical application.

Conclusions:

  • The novel compact fluorescence sensing unit provides a sensitive and non-destructive method for aflatoxin detection.
  • This technology has high potential for integration into food safety protocols along the entire food chain.
  • The developed sensing methodology contributes to improved food safety through rapid, on-site aflatoxin analysis.