Immobilized laccase: an effective biocatalyst for industrial dye degradation from wastewater

Environmental concerns due to the release of industrial wastewater contaminated with dyes are becoming more and more intense with the increasing industrialization. Decolorization of industrial effluents has become the top priority due to the continuous demand for color-free discharge into the receiving water bodies. Different dye removal techniques have been developed, among which biodegradation by laccase enzyme is competitive. Laccase, as a green catalyst, has a high catalytic activity, generates less toxic by-products, and has been extensively researched in the field of remediation of dyes. However, laccase's significant catalytic activity could only be achieved after an effective immobilization step. Immobilization helps strengthen and stabilize the protein structure of laccase, thus enhancing its functional properties. Additionally, the reusability of immobilized laccase makes it an attractive alternative to traditional dye degradation technologies and in the realistic applications of water treatment, compared with free laccase. This review has elucidated different methods and the carriers used to immobilize laccase. Furthermore, the role of immobilized laccase in dye remediation and the prospects have been discussed.