Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

1.8K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
1.8K
Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

1.1K
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pathogenesis of diffuse large B cell lymphoma proteogenotypes.

Cancer cell·2026
Same author

Enforced MYC expression directs a distinct transcriptional state during plasma cell differentiation.

Life science alliance·2025
Same author

The PRL2 phosphatase up-regulates miR-21 through activation of the JAK2/STAT3 pathway to down-regulate the PTEN tumor suppressor.

The Biochemical journal·2024
Same author

Effects of cladribine on intrathecal and peripheral B and plasma cells.

Clinical and experimental immunology·2024
Same author

PembroWM: A phase II trial to investigate the safety and efficacy of rituximab and pembrolizumab in relapsed/refractory Waldenström's Macroglobulinaemia.

British journal of haematology·2024
Same author

Long-term outcomes by bone marrow B-cell depletion from the R2W trial of bortezomib with cyclophosphamide and rituximab in Waldenstrőm macroglobulinaemia.

Leukemia·2024

Related Experiment Video

Updated: Jul 25, 2025

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
06:38

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

Published on: November 8, 2011

40.4K

Spontaneous EBV-Reactivation during B Cell Differentiation as a Model for Polymorphic EBV-Driven Lymphoproliferation.

Matthew A Care1,2, Sophie Stephenson1, Roger Owen3

  • 1Division of Haematology and Immunology, Leeds Institute of Medical Research, University of Leeds, Leeds LS9 7TF, UK.

Cancers
|June 28, 2023
PubMed
Summary

Epstein-Barr virus (EBV) reactivation in differentiating B cells creates plasma cells and B cells expressing EBV genes. These EBV-associated cells show germinal center B cell features, suggesting a link to non-switched B cells.

Keywords:
B-cellEpstein-Barr virusgerminal centrelatencyplasma cellreactivation

More Related Videos

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis
08:44

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis

Published on: September 7, 2022

2.5K
A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines
09:49

A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines

Published on: January 14, 2017

14.9K

Related Experiment Videos

Last Updated: Jul 25, 2025

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
06:38

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

Published on: November 8, 2011

40.4K
Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis
08:44

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis

Published on: September 7, 2022

2.5K
A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines
09:49

A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines

Published on: January 14, 2017

14.9K

Area of Science:

  • Immunology
  • Virology
  • Cell Biology

Background:

  • Epstein-Barr virus (EBV) drives B cell neoplasms through latent infection reactivation.
  • Polymorphous lymphoproliferative disorder (LPD) involves EBV driving B cell differentiation.
  • Spontaneous EBV reactivation models polymorphic EBV-driven LPD.

Purpose of the Study:

  • To develop an in vitro model of plasma cell (PC) differentiation from peripheral blood memory B cells.
  • To analyze EBV-associated cell populations during PC differentiation.
  • To investigate gene expression patterns in primary EBV reactivation.

Main Methods:

  • Developed an in vitro plasma cell differentiation model from memory B cells.
  • Utilized a targeted single-cell gene expression panel.
  • Analyzed eight differentiations during the PC stage.

Main Results:

  • Identified EBV-gene expressing subpopulations with PC and/or B cell features (3-28% of cells).
  • Most EBV-associated cells expressed PC genes (e.g., XBP1, MZB1), including a quiescent PC fraction.
  • Proliferative EBV-associated cells showed retained CD20 expression and IgM/IgD co-expression, while class-switched cells were quiescent PCs.

Conclusions:

  • Primary EBV reactivation patterns include germinal center B cell features.
  • EBV-transformed lymphoblastoid cell lines also exhibit GC B cell features.
  • Spontaneous EBV expansions are particularly associated with IgM+ IgD+ non-switched B cells.