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Updated: Jul 25, 2025
06:38
Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
Published on: November 8, 2011
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Spontaneous EBV-Reactivation during B Cell Differentiation as a Model for Polymorphic EBV-Driven Lymphoproliferation
Matthew A Care1,2, Sophie Stephenson1, Roger Owen3
1Division of Haematology and Immunology, Leeds Institute of Medical Research, University of Leeds, Leeds LS9 7TF, UK.
Cancers
|June 28, 2023
Summary
Epstein-Barr virus (EBV) reactivation in differentiating B cells creates plasma cells and B cells expressing EBV genes. These EBV-associated cells show germinal center B cell features, suggesting a link to non-switched B cells.
Area of Science:
- Immunology
- Virology
- Cell Biology
Background:
- Epstein-Barr virus (EBV) drives B cell neoplasms through latent infection reactivation.
- Polymorphous lymphoproliferative disorder (LPD) involves EBV driving B cell differentiation.
- Spontaneous EBV reactivation models polymorphic EBV-driven LPD.
Purpose of the Study:
- To develop an in vitro model of plasma cell (PC) differentiation from peripheral blood memory B cells.
- To analyze EBV-associated cell populations during PC differentiation.
- To investigate gene expression patterns in primary EBV reactivation.
Main Methods:
- Developed an in vitro plasma cell differentiation model from memory B cells.
- Utilized a targeted single-cell gene expression panel.
- Analyzed eight differentiations during the PC stage.
Main Results:
- Identified EBV-gene expressing subpopulations with PC and/or B cell features (3-28% of cells).
- Most EBV-associated cells expressed PC genes (e.g., XBP1, MZB1), including a quiescent PC fraction.
- Proliferative EBV-associated cells showed retained CD20 expression and IgM/IgD co-expression, while class-switched cells were quiescent PCs.
Conclusions:
- Primary EBV reactivation patterns include germinal center B cell features.
- EBV-transformed lymphoblastoid cell lines also exhibit GC B cell features.
- Spontaneous EBV expansions are particularly associated with IgM+ IgD+ non-switched B cells.
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