UAS™-A Urine Preservative for Oncology Applications

Area of Science:

• Oncology
• Biochemistry
• Molecular Diagnostics

Background:

• Liquid biopsy offers a non-invasive approach for cancer diagnostics.
• Urine is a valuable biofluid for detecting various cancers.
• Preservation of urinary analytes is essential for accurate diagnostic testing.

Purpose of the Study:

• To evaluate UAS™ as a preservative for urinary analytes.
• To assess the impact of UAS™ on cell-free DNA (cfDNA) and host cell integrity.
• To determine the efficacy of UAS™ in clinical samples from cancer patients.

Main Methods:

• Urine samples from healthy volunteers and cancer patients were analyzed.
• UAS™ performance was tested for cfDNA and cell integrity at room temperature and after freeze-thaw cycles.
• Compatibility of UAS™ with commercial isolation kits was assessed.

Main Results:

• Unpreserved urine showed cfDNA degradation and bacterial growth at room temperature.
• UAS™ prevented microbial growth and maintained cfDNA and cellular integrity for 14 days at room temperature.
• UAS™ significantly improved preservation of cfDNA and host cell integrity after freeze-thaw cycles.

Conclusions:

• UAS™ is an effective urine preservative for maintaining analyte stability.
• UAS™ supports non-invasive cancer diagnostics by preserving urinary biomarkers.
• UAS™ is compatible with standard cfDNA isolation methods, facilitating its use in clinical settings.