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Related Experiment Video

Updated: Jul 25, 2025

Simultaneous Assessment of Cardiomyocyte DNA Synthesis and Ploidy: A Method to Assist Quantification of Cardiomyocyte Regeneration and Turnover

Simultaneous Assessment of Cardiomyocyte DNA Synthesis and Ploidy: A Method to Assist Quantification of Cardiomyocyte Regeneration and Turnover

Published on: May 23, 2016

Cardiomyocyte Ploidy, Metabolic Reprogramming and Heart Repair.

Andrea Elia¹, Sadia Mohsin², Mohsin Khan^1,3

¹Center for Metabolic Disease Research, Lewis Katz School of Medicine, Temple University, Philadelphia, PA 19140, USA.

|June 28, 2023

Summary

This summary is machine-generated.

Heart muscle cells (cardiomyocytes) normally cannot divide. Metabolic changes influence cardiomyocyte division and regeneration after heart injury, offering new therapeutic avenues.

Keywords:

binucleation cardiomyocyte cell cycle metabolism ploidy

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Simultaneous Assessment of Cardiomyocyte DNA Synthesis and Ploidy: A Method to Assist Quantification of Cardiomyocyte Regeneration and Turnover

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Published on: March 22, 2017

Area of Science:

Cardiovascular Biology
Cellular Metabolism
Regenerative Medicine

Background:

Adult cardiomyocytes (CMs) are terminally differentiated and lack regenerative capacity after myocardial injury.
Developing CMs are proliferative and contribute to cardiac tissue regeneration.
CM maturation involves polyploidization and binucleation, altering function and metabolism.

Purpose of the Study:

To review changes in cardiomyocyte ploidy and nucleation during cardiac development, maturation, and injury.
To explore the role of metabolic reprogramming in CM cell cycle reentry and fate transitions.
To discuss the impact of metabolism on CM proliferation and cardiac regeneration.

Main Methods:

Literature review of studies on cardiomyocyte development, maturation, and response to injury.
Analysis of research on metabolic changes associated with CM ploidy and nucleation.
Synthesis of findings on the link between metabolism, cell cycle, and cardiac regeneration.

Main Results:

CMs transition from mononucleated diploid to polyploid/binucleated states during maturation.
Metabolic reprogramming is crucial for CM cell cycle reentry and ploidy changes.
These metabolic and cellular changes enhance cardiac structure and function post-injury.

Conclusions:

Metabolism significantly influences cardiomyocyte fate, including mononucleation/binucleation and cell cycle progression.
Understanding these metabolic-cardiomyocyte interactions is key to promoting cardiac regeneration.
Targeting metabolic pathways may offer novel strategies for treating heart disease.