Different Extraction Procedures Revealed the Anti-Proliferation Activity from Vegetable Semi-Purified Sources on...

Area of Science:

• Phytochemistry
• Oncology
• Cell Biology

Background:

• Breast cancer (BC) is a leading cause of mortality in women.
• New therapeutic compounds are critical for effective BC treatment.
• Phytochemicals possess known anti-cancer properties.

Purpose of the Study:

• To investigate the anti-proliferation potential of carrot, Calendula officinalis, and Aloe vera extracts on breast cancer and epithelial cell lines.
• To identify specific compounds responsible for anti-cancer effects.
• To explore potential treatments for triple-negative breast cancer.

Main Methods:

• Extraction of plant materials using hexane and methanol.
• Assessment of cell proliferation using proliferation assays.
• Chemical characterization of extracts using colorimetric assays, UHPLC-HRMS, and MS/MS analysis.

Main Results:

• Semi-purified extracts from carrot, Aloe vera, and Calendula officinalis specifically inhibited breast cancer cell proliferation.
• Monogalactosyl-monoacylglycerol (MGMG) was found in all extracts.
• Aloe vera extracts contained digalactosyl-monoacylglycerol (DGMG) and aloe-emodin, while Calendula extracts contained glycerophosphocholine (GPC) derivatives.

Conclusions:

• Lipid compounds, including MGMG and GPC derivatives, may be associated with the observed anti-proliferative properties.
• Calendula extract demonstrated significant inhibition of triple-negative breast cancer MDA-MB-231 cell line proliferation.
• MGMG and GPC derivatives show promise as potential therapeutic agents for specific breast cancer subtypes.