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Development and Application of Real-Time PCR-Based Screening for Identification of Omicron SARS-CoV-2 Variant

Anna Esman¹, Dmitry Dubodelov¹, Kamil Khafizov^1,2

¹Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing Russian Federation, 3a Novogireevskaya Str., 111123 Moscow, Russia.

|June 28, 2023

Abstract:

The Omicron strain is currently the main dominant variant of SARS-CoV-2, with a large number of sublineages. In this article, we present our experience in tracing it in Russia using molecular diagnostic methods. For this purpose, different approaches were used; for example, we developed multiprimer panels for RT-PCR and Sanger and NGS sequencing methods. For the centralized collection and analysis of samples, the VGARus database was developed, which currently includes more than 300,000 viral sequences.

Keywords:

Omicron RT-PCR SARS-CoV-2 variants surveillance

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