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Quantification of Hypopigmentation Activity In Vitro
Published on: March 6, 2019
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Syringetin Promotes Melanogenesis in B16F10 Cells
1Jeju Inside Agency and Cosmetic Science Center, Department of Chemistry and Cosmetics, Jeju National University, Jeju 63243, Republic of Korea.
International Journal of Molecular Sciences
|June 28, 2023
Summary
Syringetin, found in red grapes, stimulates melanin production by increasing tyrosinase activity and related protein expression in skin cells. This natural compound is safe for topical use, offering potential for cosmetic and medical applications.
Area of Science:
- Biochemistry
- Dermatology
- Pharmacology
Background:
- Syringetin, a myricetin derivative from natural sources, exhibits various pharmacological effects.
- Its impact on melanogenesis and the underlying molecular mechanisms were previously unexplored.
- Melanogenesis is crucial for skin pigmentation and protection.
Purpose of the Study:
- To investigate the effect of syringetin on melanogenesis in B16F10 melanoma cells.
- To elucidate the molecular pathways involved in syringetin-induced melanogenesis.
- To assess the safety of syringetin for topical application.
Main Methods:
- Treatment of B16F10 melanoma cells with varying concentrations of syringetin.
- Measurement of melanin production and tyrosinase activity.
- Analysis of protein expression for MITF, tyrosinase, TRP-1, TRP-2, ERK, PI3K/Akt, p38, JNK, PKA, GSK3β, and β-catenin.
- Assessment of signaling pathway activation via phosphorylation.
- Primary skin irritation test on human volunteers.
Main Results:
- Syringetin significantly increased melanin production and tyrosinase activity in a dose-dependent manner.
- It upregulated MITF, tyrosinase, TRP-1, and TRP-2 protein levels.
- Syringetin modulated key signaling pathways including ERK, PI3K/Akt, p38, JNK, PKA, and GSK3β/β-catenin.
- Topical application of syringetin showed no adverse skin reactions in human volunteers.
Conclusions:
- Syringetin effectively stimulates melanogenesis through complex signaling pathways, including the GSK3β/β-catenin pathway.
- It represents a promising agent for cosmetic applications and treating hypopigmentation disorders.
- Syringetin demonstrates a favorable safety profile for skin application.
