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Fabrication of Zero Mode Waveguides for High Concentration Single Molecule Microscopy

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Study and Optimization of a Micro-Structured Waveguiding and Fluorescent Sol-Gel Architecture.

Ibtihel Marzouk^1,2, David Riassetto¹, Alain Morand²

¹LMGP, Grenoble INP, CNRS, University Grenoble Alpes, F-38000 Grenoble, France.

Molecules (Basel, Switzerland)

|June 28, 2023

Summary

Researchers developed a novel fluorescent micro-structured architecture on glass using sol-gel processing. This integrated optics component efficiently couples light into and out of channel waveguides, paving the way for advanced photonic devices.

Keywords:

channel waveguide diffraction grating fluorescence intensity sol-gel processing

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Area of Science:

Integrated optics and photonics
Materials science
Sol-gel processing

Background:

Channel waveguides with diffraction gratings are crucial for integrated optics.
Existing methods often face limitations in fabrication and efficiency.

Purpose of the Study:

To report the first fluorescent micro-structured architecture on glass using sol-gel processing.
To demonstrate efficient light coupling and propagation within the waveguide for fluorescence measurements.

Main Methods:

Sol-gel processing utilizing a titanium oxide-based photoresist.
Single-step photolithography for imprinting channel waveguides and diffraction gratings.
Optical characterization and simulation of the fabricated architecture.

Main Results:

Reproducible and uniform grating/waveguide architectures fabricated over large dimensions.
Efficient coupling of light at excitation and emission wavelengths of the ruthenium complex fluorophore (Rudpp).
Effective propagation and extraction of fluorescence signals with minimal losses and photobleaching.

Conclusions:

The developed sol-gel architecture is suitable for efficient waveguiding and fluorescence detection.
This technology offers a promising foundation for integrating photonic devices with microfluidic platforms.
Further development could enable real-time fluorescence measurements in liquid media.