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Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells
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The peroxisome proliferator-activated receptor γ coactivator-1 alpha rs8192678 (Gly482Ser) variant and hepatitis B

Karima Abounouh1,2, Ikram-Allah Tanouti1, Ahd Ouladlahsen3

  • 1Virology Unit, Viral Hepatitis Laboratory, Institut Pasteur du Maroc, Casablanca, Morocco.

Infectious Diseases (London, England)
|June 28, 2023
PubMed

Insights

The PPARGC1A rs8192678 variant is associated with spontaneous clearance of hepatitis B virus infection. This genetic factor may help predict acute HBV infection outcomes in Moroccan patients.

Area of Science:

  • Genetics
  • Hepatology
  • Virology

Background:

  • Chronic hepatitis B virus (CHB) infection remains a significant global health challenge with no definitive cure.
  • Host genetic factors influencing HBV infection development are not fully understood.
  • The PPARGC1A gene, previously linked to liver diseases, is investigated for its role in HBV regulation.

Purpose of the Study:

  • To investigate the association of the PPARGC1A rs8192678 (Gly482Ser) variant with spontaneous HBV clearance.
  • To determine if this variant influences the progression of chronic hepatitis B in Moroccan patients.
  • To explore the potential of rs8192678 as a predictive marker for HBV infection outcomes.

Main Methods:

  • Genotyping of the rs8192678 single nucleotide polymorphism (SNP) using TaqMan allelic discrimination.
  • Study cohort comprised 292 CHB patients and 181 individuals with spontaneous HBV clearance.
  • Statistical analysis to explore associations with spontaneous clearance and CHB progression, including liver enzymes and viral load.

Main Results:

  • Individuals with CT and TT genotypes of rs8192678 showed a significantly higher likelihood of spontaneous HBV clearance (p < 0.0001).
  • Carriage of the mutant allele T was strongly associated with spontaneous clearance (OR=0.51, P=2.68E-06).
  • No significant association was found between rs8192678 genotypes and CHB progression, ALT, AST, or HBV viral loads.

Conclusions:

  • The PPARGC1A rs8192678 variant appears to modulate acute HBV infection, influencing spontaneous clearance.
  • This SNP may serve as a potential predictive marker for HBV infection outcomes within the Moroccan population.
  • Further research is warranted to elucidate the precise mechanisms of PPARGC1A in HBV pathogenesis.
Abstract

Keywords:
HBVPPARGC1Achronic hepatitis Bclearancevariant

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