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Investigation of miR-26a-5p and miR-19a-3p expression levels in angiographically confirmed coronary artery disease
Neslihan Coban1, Aycan F Erkan2, Aybike Sena Ozuynuk-Ertugrul1,3
1Department of Genetics, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
Background:
MicroRNAs have been found to have an essential role in cardiovascular diseases. In previous experiments, the changed expressions of miR-26a-5p and miR-19a-3p were confirmed in patients with severe coronary atherosclerosis by miRNA microarrays. However, the role of two miRNAs in coronary artery diseases (CAD) still needs to be investigated further. Our current study aimed to analyse two miRNAs in angiographically confirmed CAD and non-CAD with insignificant coronary stenosis. This study aimed to identify the potential diagnostic value of circulating miRNA with CAD.
Methods:
The CAD patients (n = 50) and non-CAD controls (n = 43) were studied. miRNAs (miR-26a-5p and miR-19a-3p) were quantified by TaqMan miRNA assays using real-time PCR. We subsequently assessed the diagnostic value of the miRNAs and correlations of miRNA with clinical parameters. Target prediction tools were utilised to identify miRNA target genes.
Results:
The expression of miR-26a-5p was significantly increased in CAD compared to non-CAD controls (p < 0.05). Tertile groups were formed according to the expression levels of miRNAs, and high expression tertile (T3) was compared with low expression tertile (T1). It was found that CAD presence was more prevalent in T3 of miR-26a-5p, and the frequency of diabetes was higher in T3 of miR-19a-3p. There were significant correlations between miRNAs and diabetes risk factors such as HbA1c, glucose levels, and BMI (p < 0.05).
Conclusions:
Our findings show that miR-26a-5p expression is altered in CAD presence while miR-19a-3p expression is different in diabetes. Both miRNAs are closely related to risk factors of CAD, therefore, could be therapeutic targets for CAD treatment.
Insights
Circulating miR-26a-5p levels are elevated in coronary artery disease (CAD) patients. Both miR-26a-5p and miR-19a-3p correlate with diabetes risk factors, suggesting potential as therapeutic targets for CAD.
Area of Science:
- Cardiovascular Biology
- Molecular Diagnostics
- Biomarker Discovery
Background:
- MicroRNAs (miRNAs) play a crucial role in cardiovascular diseases.
- Previous studies indicated altered miR-26a-5p and miR-19a-3p expression in severe coronary atherosclerosis.
- The specific roles of these miRNAs in coronary artery disease (CAD) require further investigation.
Purpose of the Study:
- To analyze the expression of miR-26a-5p and miR-19a-3p in patients with angiographically confirmed CAD.
- To evaluate the potential diagnostic value of these circulating miRNAs for CAD.
- To explore the correlation of miRNA expression with clinical parameters and risk factors.
Main Methods:
- Quantification of miR-26a-5p and miR-19a-3p using TaqMan miRNA assays and real-time PCR.
- Comparison of miRNA expression between 50 CAD patients and 43 non-CAD controls.
- Assessment of diagnostic value and correlation with clinical parameters, including diabetes risk factors.
Main Results:
- miR-26a-5p expression was significantly increased in CAD patients compared to controls (p < 0.05).
- Higher miR-26a-5p expression correlated with CAD presence, while higher miR-19a-3p expression was linked to increased diabetes frequency.
- Both miRNAs showed significant correlations with diabetes risk factors like HbA1c, glucose levels, and BMI (p < 0.05).
Conclusions:
- miR-26a-5p expression is altered in the presence of CAD.
- miR-19a-3p expression differs in individuals with diabetes, and both miRNAs are associated with CAD risk factors.
- These miRNAs represent potential therapeutic targets for CAD treatment.
