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Updated: Jul 25, 2025

Microplot Design and Plant and Soil Sample Preparation for 15Nitrogen Analysis
Published on: May 10, 2020
Poly-γ-glutamic acid improved biological nitrogen fixation, water-nitrogen productivity, and nitrate residue in
Bo Jing1, Wenjuan Shi1, Lu Liu1
1State Key Laboratory of Eco-Hydraulics in Northwest Arid Region of China, Xi'an University of Technology, Xi'an, China.
Background:
Poly-γ-glutamic acid (γ-PGA) can promote crop growth and improve soil properties efficiently. However, the optimal application rate of γ-PGA in legume/non-legume intercropping systems is still unclear. A potted experiment was conducted to investigate the effects of five γ-PGA rates (0%, 0.1%, 0.2%, 0.3%, and 0.4%, represented by CK, P1, P2, P3, and P4, respectively) on biological nitrogen (N) fixation (BNF), water-N productivity, and nitrate distribution in a cotton/soybean intercropping system.
Results:
The results showed that the growth indicators (plant height, stem diameter, leaf area index, root dry weight, root length) of cotton and soybean increased first and then decreased with increasing γ-PGA rates, and all growth indicators of cotton and soybean showed peaks in P3 and P2 treatments. The stable 15 N isotope method indicated that γ-PGA promoted the BNF capacity of soybean and soil. In particular, the percentage of N derived from the atmosphere (Ndfa) in soybean reached 61.94% in the P2 treatment. Poly-γ-glutamic acid improved the water-N productivity, and the total N partial factor productivity (NPFP) and water productivity (WP) in P3 treatment increased by 23.80% and 43.86% compared with the CK treatment. The γ-PGA mitigation of potential nitrate residue also decreased first and then increased with increasing γ-PGA rates.
Conclusion:
Multivariate regression analysis showed that 0.22% of the optimal γ-PGA application rate could obtain a higher yield and water-N productivity in cotton/soybean intercropping system simultaneously. © 2023 Society of Chemical Industry.
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