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Nutrient Supplementation Improves Contact Lens-Induced Corneal Cell Damage Based on a SIRC Cellular Model.
Wan-Hsin Chang1, Meng-Chun Tsai, Pei-Yi Liu
1R&D Center (W.-H.C., M.-C.T., P.-Y.L., C.-J.L.), Yung Sheng Optical Co, Taichung, Taiwan; and Department of Chemistry (Y.-H.H.H.), Tunghai University, Taichung, Taiwan.
Eye & Contact Lens
|June 28, 2023
Summary
Nutrient supplementation, including vitamins and amino acids, can aid corneal cell repair after contact lens damage. Specific nutrients like Vitamin B2 and taurine show promise in improving ocular surface health.
Area of Science:
- Ophthalmology
- Cell Biology
- Biochemistry
Background:
- Long-term contact lens wear can compromise ocular surface integrity and corneal cell metabolism.
- Vitamins and amino acids are crucial for maintaining normal eye physiological functions.
Purpose of the Study:
- To investigate the impact of vitamin and amino acid supplementation on corneal cell repair following contact lens-induced damage.
- To evaluate the protective effects of specific nutrients on corneal cells under simulated contact lens conditions.
Main Methods:
- Utilized high-performance liquid chromatography to determine nutrient levels in cell culture media.
- Employed the MTT assay to assess corneal cell viability.
- Established a rabbit cornea cellular model to mimic contact lens-induced keratopathy.
Main Results:
- Corneal cell viability was significantly higher (83.3%) in the high water content (78%) contact lens group compared to the low water content (38%) group (51.6%).
- A 32.0% difference in cell viability highlights the correlation between contact lens water content and corneal health.
Conclusions:
- Supplementation with Vitamin B2, Vitamin B12, asparagine, and taurine demonstrated potential benefits for mitigating contact lens-induced corneal damage.
- These findings suggest a therapeutic role for specific nutrients in managing ocular surface complications associated with contact lens use.
