Related Experiment Video
Updated: Jul 25, 2025
Quantifying Human Monocyte Chemotaxis In Vitro and Murine Lymphocyte Trafficking In Vivo
Published on: October 30, 2017
Protocol for in vitro assessment of human monocyte transendothelial migration using a high-throughput live cell
Ségolène Ladaigue1, Vincent Paget2, Anne-Charlotte Lefranc2
1Institut de Radioprotection et de Sûreté Nucléaire (IRSN), PSE-SANTE/SERAMED/LRMed, 92260 Fontenay-aux-Roses, France; Sorbonne University, Doctoral College, 75005 Paris, France.
Abstract:
In vitro modeling of the different steps of immune cell recruitment is essential to decipher the role of endothelial cells in this process. Here, we present a protocol for the assessment of human monocyte transendothelial migration using a live cell imaging system. We describe steps for culture of fluorescent monocytic THP-1 cells and chemotaxis plate preparation with HUVEC monolayers. We then detail real-time analysis using the IncuCyte® S3 live-cell imaging system, image analysis, and assessment of transendothelial migration rates. For complete details on the use and execution of this protocol, please refer to Ladaigue et al.1.
