A novel multimode biosensor for sensitive detection of AFB₁ in food based on Mxenes nano enzymes

In this work, Ti₃C₂ nano-enzymes (Ti₃C₂ NEs) materials with simulated peroxidase activity and fluorescence quenching properties were prepared. Then Ti₃C₂ NEs was functionalized using 6-carboxyfluorescein (FAM) labeled Aflatoxin B₁ (AFB₁) aptamers to construct a novel multimode nano enzyme biosensor for the detection of AFB₁ in peanuts. Based on the fluorescence quenching characteristics and the superior simulated peroxidase activity of Ti₃C₂ NE_S and the specific binding of the aptamer to AFB₁, the sensitive and rapid fluorescence/colorimetric/smart phone detection of AFB₁ have been achieved, with detection limits of 0.09 ng mL^-1, 0.61 ng mL^-1 and 0.96 ng mL^-1, respectively. The analytical method provided can not only detect AFB₁ in multiple modes, but also has a wider detection range, lower limit of detection (LOD) and better recovery rate, and can achieve on-site accurate detection of AFB₁ content in peanuts, which has great application potential in the field of food quality testing.