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Related Concept Videos

Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

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The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
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Anaphase Promoting Complex00:50

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The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
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Retrovirus Life Cycles01:10

Retrovirus Life Cycles

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Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
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Subviral Agents01:29

Subviral Agents

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Subviral agents are infectious entities that resemble viruses but lack one or more viral components, such as a capsid or essential replication machinery. These agents include viroids, prions, and satellites, each possessing distinct structural and functional characteristics that influence their mode of infection and replication.Viroids are the simplest subviral agents, consisting of circular, single-stranded RNA molecules without a protein coat. They exclusively infect plants, relying entirely...
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Antiarrhythmic Drugs: Class II Agents as β-Adrenergic Blockers01:24

Antiarrhythmic Drugs: Class II Agents as β-Adrenergic Blockers

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Adrenergic stimulation generally impacts cardiac rate and rhythm. Specifically, stimulation of the β-adrenoceptors triggers an increase in intracellular calcium ion influx and pacemaker currents, which may cause arrhythmias. Catecholamines like adrenaline also demonstrate β2-adrenoceptor-mediated hypokalemia, impacting cardiac action potential and disrupting the normal cardiac rhythm. Class II antiarrhythmic drugs are β-adrenoceptor antagonists or β-blockers, which...
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siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
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Related Experiment Video

Updated: Jul 25, 2025

Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2
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Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2

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Anthracyclines inhibit SARS-CoV-2 infection.

Zhen Wang1, Qinghua Pan2, Ling Ma3

  • 1Lady Davis Institute, Jewish General Hospital, Montreal, Quebec, Canada; Department of Medicine, McGill University, Montreal, Quebec, Canada.

Virus Research
|June 28, 2023
PubMed
Summary

Researchers screened microbial metabolites for new SARS-CoV-2 inhibitors. Aclarubicin, an anthracycline, showed promise by reducing viral gene expression, offering potential for COVID-19 treatment and future coronavirus preparedness.

Keywords:
AnthracyclineInterferonMicrobial metabolitesSARS-CoV-2

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Related Experiment Videos

Last Updated: Jul 25, 2025

Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2
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Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds
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Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
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Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

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Area of Science:

  • Virology
  • Drug Discovery
  • Antimicrobial Research

Background:

  • Existing SARS-CoV-2 drugs face limitations like resistance and usage restrictions.
  • There is a need for novel antiviral agents to combat COVID-19 and prepare for future coronavirus threats.
  • Repurposing existing drugs or discovering new ones is crucial for pandemic preparedness.

Purpose of the Study:

  • To screen microbial metabolites for novel SARS-CoV-2 inhibitors.
  • To identify compounds effective against SARS-CoV-2, including drug-resistant variants.
  • To explore the potential of anthracyclines as antiviral agents against coronaviruses.

Main Methods:

  • Screening of a microbial metabolite library against SARS-CoV-2.
  • Generation of a recombinant SARS-CoV-2 Delta variant with a nano-luciferase reporter.
  • Measurement of viral inhibition using half maximal inhibitory concentration (IC50) assays.

Main Results:

  • Six compounds inhibited SARS-CoV-2 with IC50 below 1 μM.
  • Aclarubicin significantly reduced viral RNA-dependent RNA polymerase (RdRp)-mediated gene expression.
  • Other anthracyclines demonstrated antiviral activity by upregulating interferon and antiviral genes.

Conclusions:

  • Anthracyclines, commonly used anti-cancer drugs, show potential as novel SARS-CoV-2 inhibitors.
  • Aclarubicin's mechanism involves direct inhibition of viral gene expression.
  • This study contributes to the search for new therapeutics against SARS-CoV-2 and other coronaviruses.