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Cell Culture

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media

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Related Experiment Video

Updated: Jul 25, 2025

Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures

Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures

Published on: August 18, 2023

[Progress on three-dimensional cell culture technology and their application].

Xiaoqin Lu¹, Xiaofeng Liu¹, Hao Zhong¹

¹College of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, P. R. China.

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi = Journal of Biomedical Engineering = Shengwu Yixue Gongchengxue Zazhi

|June 28, 2023

Summary

This summary is machine-generated.

Three-dimensional (3D) cell culture models mimic in vivo environments, offering a more accurate system than 2D cultures. These advanced models are ideal for studying drug effects and cancer cell metastasis.

Keywords:

Active substance Application Functional evaluation In vitro culture Three-dimensional cell culture

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Last Updated: Jul 25, 2025

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Area of Science:

Biotechnology
Cell Biology
Biomedical Engineering

Background:

Traditional two-dimensional (2D) cell cultures do not fully replicate the complex in vivo microenvironment.
Three-dimensional (3D) cell culture models offer a more physiologically relevant system for studying cellular behavior.
Understanding cellular responses in a 3D context is crucial for accurate drug screening and disease modeling.

Approach:

This review compares cellular responses in 2D versus 3D culture systems.
It details the methodologies for establishing 3D cell culture models.
The paper summarizes current applications and future prospects of 3D cell culture technology.

Key Points:

3D cell models exhibit distinct cellular behaviors (attachment, migration, mitosis, apoptosis) compared to 2D cultures.
These models provide a superior platform for evaluating dynamic pharmacological effects of substances.
3D cultures are valuable for investigating cancer cell metastasis and intestinal absorption processes.

Conclusions:

3D cell culture technology presents a significant advancement over 2D methods for in vitro research.
Its applications in tumor and intestinal absorption models are rapidly expanding.
The future of 3D cell models shows great promise for active substance evaluation and screening.