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Related Experiment Video

Updated: Jul 25, 2025

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture
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WAVE-based intensified perfusion cell culture for fast process development.

Zhe Lang1, Shaofan Yan1, Qingqing Xiong1

  • 1Cell Culture Process Development (CCPD), WuXi Biologics, 288 Fute Zhong Road, Waigaoqiao Free Trade Zone, Shanghai, 200131, China.

Biotechnology Letters
|June 29, 2023
PubMed
Summary

This study shows the WAVE 25 rocking bioreactor is feasible for intensified perfusion culture (IPC) of monoclonal antibodies (mAbs) in Chinese hamster ovary (CHO) cells, offering comparable performance and improved quality.

Keywords:
CHO cellsCell cultureIntensified perfusionMonoclonal antibody (mAb)Wave perfusion

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Area of Science:

  • Biotechnology
  • Bioprocessing
  • Cell Culture Engineering

Background:

  • Intensified Perfusion Culture (IPC) is crucial for efficient monoclonal antibody (mAb) production.
  • Traditional stirred tank bioreactors are commonly used for IPC.
  • Exploring alternative bioreactor systems can enhance process flexibility and efficiency.

Purpose of the Study:

  • To evaluate the feasibility of using a WAVE 25 rocking bioreactor system in an intensified perfusion culture (IPC) mode.
  • To assess its suitability for monoclonal antibody (mAb) production using a Chinese hamster ovary (CHO) cell line.
  • To compare its performance against a standard bench-top glass bioreactor IPC.

Main Methods:

  • Utilized a disposable perfusion bag with a floating membrane for the IPC process.
  • Employed an automated filter switching system for continuous clarification of harvested culture fluid.
  • Compared cell culture performance, product titer, and quality metrics with a conventional glass bioreactor IPC.

Main Results:

  • Cell culture performance and product titer trends were similar to the conventional glass bioreactor.
  • Purity-related quality attributes were slightly improved compared to the typical run.
  • Continuous clarification enabled suitability for downstream continuous chromatography.

Conclusions:

  • Demonstrated the feasibility of the WAVE-based rocking bioreactor for the N-stage IPC process.
  • The system offers increased flexibility for adopting IPC strategies.
  • The rocking bioreactor is a viable alternative to stirred tank bioreactors for perfusion culture in biopharmaceutical manufacturing.