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Updated: Jul 25, 2025
Fluorescence detection methods for microfluidic droplet platforms
Published on: December 10, 2011
Continuous Preparation of Semiconducting Polymer Nanoparticles with Varied Sizes for Online Fluorescence Sensing via
Ruixuan Hu1, Xiaolong Li2, Jian Xu2
1School of Chemistry and Molecular Engineering, Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, East China Normal University, Dongchuan Road 500, Shanghai 200241, China.
Abstract:
Microfluidic chips are in critical demand for emerging applications in material synthesis and biosensing. Herein, we relied on ultrafast laser-processing technology to fabricate a three-dimensional (3D) microfluidic chip, in which semiconducting polymer nanoparticles (SPNs) were continuously synthesized with tunable size and SPN-involved online fluorescence sensing was implemented. A homogeneous distribution of SPNs can be readily realized due to the efficient mixing and powerful vortices of the 3D microfluidic chip, which prevents SPNs from aggregating throughout the synthesis process. Moreover, in the optimized conditions, we unveiled unique SPNs with an ultrasmall particle size (<3 nm) and good monodispersity. By integrating with the high-performance fluorescence of SPNs and 3D microfluidic chip, we further developed an online sensing platform for ratiometric fluorescence assays of H2O2 and oxidase-catalyzed substrates (e.g., glucose), in which a composite of SPNs and neutral red (NR) (SPNs/NR) was used as the mediator. The limit of detection (LOD) for H2O2 is 0.48 μM, and the LOD for glucose is 3.33 μM via the presented platform. This 3D microfluidic synthesis-and-sensing platform provides a new avenue for the facile production of nanoparticles and offers exciting prospects in the field of online sensing biomarkers.
