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Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

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Related Experiment Video

Updated: Jul 25, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Spatially resolved single-cell translatomics at molecular resolution.

Hu Zeng^1,2, Jiahao Huang^1,2, Jingyi Ren^1,2

¹Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Science (New York, N.Y.)

|June 29, 2023

Summary

This summary is machine-generated.

Researchers developed RIBOmap, a new method to map messenger RNA (mRNA) translation in single cells. This technique reveals cell-specific gene expression patterns and localized translation in the brain.

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Published on: April 30, 2011

Related Experiment Videos

Last Updated: Jul 25, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Author Spotlight: Investigating mRNA Spatial Distribution in Drosophila Muscle Tissue

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Published on: September 8, 2023

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

Published on: April 30, 2011

Area of Science:

Molecular Biology
Genetics
Neuroscience

Background:

Precise control of messenger RNA (mRNA) translation is vital for gene regulation.
Studying mRNA translation at transcriptomic scale with spatial and single-cell resolution is challenging.

Purpose of the Study:

Develop a novel method for in situ profiling of the cellular translatome.
Enable systematic study of mRNA translation with spatial and single-cell resolution.

Main Methods:

Developed ribosome-bound mRNA mapping (RIBOmap), a highly multiplexed 3D in situ profiling technique.
Applied RIBOmap to profile 981 genes in HeLa cells and 5413 genes in mouse brain tissues.

Main Results:

RIBOmap revealed cell cycle-dependent translational control and colocalization of functional gene modules in HeLa cells.
Generated spatially resolved single-cell translatomic profiles for 119,173 mouse brain cells.
Identified cell type-specific and brain region-specific translational regulation, including oligodendrocyte maturation.
Detected widespread localized translation in neuronal and glial cells within intact brain tissue.

Conclusions:

RIBOmap is a powerful tool for studying the translatome at high resolution.
The method provides new insights into spatial and single-cell translational regulation in complex tissues.
Revealed dynamic translational remodeling during oligodendrocyte development and localized translation in brain cells.