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Related Experiment Video

Updated: Jul 25, 2025

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria
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Mycobacterium tuberculosis H37Rv enolase (Rv1023)- expression, characterization and effect of host dependent

Ajay Kumar1, Vishant Mahendra Boradia1, Apurwa Mahajan2

  • 1Department of Biotechnology National Institute of Pharmaceutical Education and Research (NIPER), Phase X, Sector 67, SAS Nagar, Punjab, 160062, India.

Biochimie
|June 29, 2023
PubMed
Summary

Mycobacterium tuberculosis enolase

Keywords:
BiofilmEnolaseEnzyme activityMycobacterium tuberculosisProtein expressionProtein multifunctionality

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Area of Science:

  • Biochemistry
  • Microbiology
  • Enzymology

Background:

  • Mycobacterium tuberculosis enolase is a key glycolytic enzyme.
  • It links glycolysis to the TCA pathway and has roles in invasion and biofilms.
  • PEP depletion is linked to drug-resistant, non-replicating bacteria.

Purpose of the Study:

  • To express and characterize Mycobacterium tuberculosis enolase.
  • To investigate the impact of expression host on enolase function.
  • To confirm enolase's role in biofilm formation.

Main Methods:

  • Expression and characterization of Mycobacterium tuberculosis enolase in Mtb H37Ra and E. coli.
  • Analysis of post-translational modifications.
  • Assessment of enzyme activity and biofilm formation.

Main Results:

  • Expression host significantly impacts enolase activity and alternate functions.
  • Subtle differences in post-translational modifications were observed.
  • Enolase confirmed to play a role in Mycobacterium tuberculosis biofilm formation.

Conclusions:

  • Choice of expression host is critical for functional characterization of Mycobacterium tuberculosis enolase.
  • Understanding these differences aids in developing novel anti-mycobacterials.
  • Inhibiting enolase presents a potential strategy against Mtb biofilms.