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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Related Experiment Video

Updated: Jul 25, 2025

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
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DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

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Creating an ultra-sensitive detection platform for monkeypox virus DNA based on CRISPR technology.

Tong Jiang1,2, Ge Li1,2, Runde Liu1,2

  • 1The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.

Journal of Medical Virology
|June 30, 2023
PubMed
Summary

This study presents a new CRISPR-based molecular diagnostic strategy for detecting monkeypox virus (MPXV). The developed method offers a cost-effective and accurate point-of-care alternative to quantitative PCR (qPCR) for MPXV detection.

Keywords:
CRISPR dual systemPOCTdiagnosismonkeypox virusrecombinase polymerase amplification

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Area of Science:

  • Molecular Biology
  • Virology
  • Biotechnology

Background:

  • The global monkeypox virus (MPXV) outbreak necessitates rapid and accessible diagnostic tools.
  • Quantitative PCR (qPCR) is the current standard but is limited by cost and infrastructure requirements.
  • CRISPR technology offers potential for point-of-care pathogen detection.

Purpose of the Study:

  • To develop and evaluate a CRISPR-based molecular diagnostic strategy for MPXV detection.
  • To create a cost-effective and accessible alternative to qPCR for MPXV diagnosis.
  • To assess the clinical applicability of the developed CRISPR assay.

Main Methods:

  • Utilized Cas12a and Cas13a enzymes for detecting MPXV-specific F3L and B6R genes.
  • Developed two protocols: a 2-step method and a single-tube method combining CRISPR and recombinase polymerase amplification.
  • Assessed sensitivity, specificity, and cross-reactivity with other viruses and bacteria.

Main Results:

  • The CRISPR-based assay detected MPXV down to 10 copies/μL.
  • Demonstrated high specificity with no cross-reactivity against other poxviruses, pseudoviruses, and bacteria.
  • Mock positive samples showed satisfactory concordance with qPCR, indicating clinical applicability.

Conclusions:

  • The developed CRISPR Dual System provides a reliable and sensitive molecular diagnostic strategy for MPXV detection.
  • This method offers a promising alternative for resource-limited settings.
  • The study contributes a valuable tool for managing MPXV outbreaks.