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Updated: Jul 25, 2025
07:46
Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
Published on: October 1, 2016
11.8K
Single-cell omic molecular profiling using capillary electrophoresis-mass spectrometry
Ketki Bagwe1, Noah Gould1, Kendall R Johnson1
1Barnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology, Northeastern University, 360 Huntington Ave., Boston, MA, 02115, United States.
Trends in Analytical Chemistry : TRAC
|June 30, 2023
Summary
Single-cell analysis using capillary electrophoresis coupled with mass spectrometry (CE-MS) reveals cellular heterogeneity. This technique advances proteomic and metabolomic profiling for understanding biological processes and pathologies.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Cell Biology
Background:
- Cellular heterogeneity, arising from molecular differences, is key to biological phenomena and diseases.
- Bulk-cell sampling methods obscure crucial cell-to-cell variations.
- Analyzing individual cells is essential for a deeper biological understanding.
Purpose of the Study:
- To review the application of capillary electrophoresis coupled with mass spectrometry (CE-MS) for single-cell proteomics and metabolomics.
- To highlight recent advancements in CE-MS techniques for single-cell analysis.
Main Methods:
- Capillary electrophoresis coupled with mass spectrometry (CE-MS) for single-cell analysis.
- Focus on sample preparation, separation, MS acquisition, and data analysis.
Main Results:
- CE-MS is a powerful technique for single-cell proteomic and metabolomic profiling.
- Recent advances have improved the capabilities of CE-MS for analyzing individual cells.
Conclusions:
- CE-MS enables detailed analysis of cellular heterogeneity at the single-cell level.
- This technique is vital for advancing research in biology and pathology.
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