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Radioimmunoassay of human tissue factor
Thrombosis Research
|August 1, 1986
Summary
Researchers purified tissue factor apoprotein (TF-AP) and developed a sensitive radioimmunoassay (RIA) to measure it. Elevated TF-AP levels were found in acute non-lymphoid leukemia patients with disseminated intravascular coagulation (DIC).
Area of Science:
- Biochemistry
- Hematology
- Immunology
Background:
- Tissue factor (TF) plays a crucial role in hemostasis and thrombosis.
- Understanding TF apoprotein (TF-AP) levels can provide insights into coagulation disorders.
Purpose of the Study:
- To purify TF-AP from human placenta.
- To develop a sensitive radioimmunoassay (RIA) for TF-AP quantification.
- To investigate TF-AP levels in patients with acute non-lymphoid leukemia (ANLL), particularly those with disseminated intravascular coagulation (DIC).
Main Methods:
- Purification of TF-AP using acetone delipidation, DOC extraction, Sephacryl S-300 chromatography, and PAGE.
- Development of a radioimmunoassay (RIA) using an antibody against purified TF-AP.
- Quantification of immunoreactive TF-AP (IR-TFR) in leukemic leukocytes from ANLL patients.
Main Results:
- TF-AP was purified 72,000-fold to homogeneity with an apparent molecular weight of 54,000.
- The developed RIA demonstrated high sensitivity (100 pg/ml) and reproducibility.
- ANLL patients with DIC exhibited significantly higher IR-TFR levels (157.9 ng/10(8) cells) compared to those without DIC (37.1 ng/10(8) cells).
Conclusions:
- A reliable method for TF-AP purification and quantification was established.
- Elevated TF-AP levels are associated with ANLL, especially in the presence of DIC.
- TF-AP may serve as a potential biomarker for DIC in ANLL patients.