A pressure-colorimetric multimode system with photothermal activated multiple rolling signal amplification for...

Utilizing the photothermal effect to activate enzyme activity, realize signal conversion and amplification show promising prospects in biosensing. Herein, a pressure-colorimetric multi-mode bio-sensor was proposed through the multiple rolling signal amplification strategy of photothermal control. Under NIR light radiation, the Nb₂C MXene labeled photothermal probe caused notable temperature elevation on a multi-functional signal conversion paper (MSCP), leading to decomposition of thermal responsive element and in-situ formation of Nb₂C MXene/Ag-S_x hybrid. The generation of Nb₂C MXene/Ag-S_x hybrid accompanied with valid color change from pale yellow to dark brown on MSCP. Moreover, the Ag-S_x as a signal amplification element enhanced the NIR light absorption to further improve the photothermal effect of Nb₂C MXene/Ag-S_x thereby induce cyclic in situ production of Nb₂C MXene/Ag-S_x hybrid with rolling enhanced photothermal effect. Subsequently, the continuously enhanced photothermal effect rolling activated catalase-like activity of Nb₂C MXene/Ag-S_x, which accelerated the decomposition of H₂O₂ and promoted the pressure elevation. Therefore, the rolling-enhanced photothermal effect and rolling activated catalase-like activity of Nb₂C MXene/Ag-S_x considerately amplified the pressure and color change. Making full use of multi-signal readout conversion and rolling signal amplification, accurate results can be obtained in a short time, whether in the laboratory or in the patient's homes.