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Related Experiment Video

Updated: Jul 25, 2025

Developing 3D Organized Human Cardiac Tissue within a Microfluidic Platform
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Developing 3D Organized Human Cardiac Tissue within a Microfluidic Platform

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Vascularized hiPSC-derived 3D cardiac microtissue on chip.

Ulgu Arslan1, Marcella Brescia1, Viviana Meraviglia1

  • 1Department of Anatomy and Embryology, Leiden University Medical Centre, 2333ZC Leiden, the Netherlands.

Stem Cell Reports
|June 30, 2023
PubMed
Summary

We created a vascularized human cardiac microtissue model using stem cells in an organ-on-chip system. This model shows improved cell communication and inflammatory responses, useful for drug testing.

Keywords:
Heart-on-chipOrgan-on-chipcardiac microtissuecell-cell interactionhiPSC-derived cardiomyocyteshiPSCshuman induced pluripotent stem cellsvascularization

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Area of Science:

  • Biomedical Engineering
  • Stem Cell Biology
  • Cardiovascular Research

Background:

  • Functional vasculature is critical for cardiac health, supplying nutrients and removing waste.
  • Existing in vitro models often lack complex, functional vascular networks.
  • Human induced pluripotent stem cells (hiPSCs) offer a promising source for generating cardiac and vascular cells.

Purpose of the Study:

  • To develop an in vitro vascularized human cardiac microtissue model using hiPSCs.
  • To investigate spontaneous vascular network formation and anastomosis within the microtissue.
  • To assess the impact of vascularization on cell communication and inflammatory responses.

Main Methods:

  • Co-culturing hiPSC-derived cardiac microtissues with vascular cells in a fibrin hydrogel within a microfluidic chip.
  • Utilizing continuous perfusion to promote vascular network development and anastomosis.
  • Analyzing vascular network formation, lumenization, and cell-cell communication.

Main Results:

  • Spontaneous formation of lumenized and interconnected vascular networks within and around cardiac microtissues.
  • Fluid flow significantly enhanced vessel density and promoted anastomosis, forming hybrid vessels.
  • Vascularization improved endothelial cell-cardiomyocyte communication via paracrine factors (e.g., nitric oxide).
  • Enhanced inflammatory responses were observed in the vascularized cardiac microtissues.

Conclusions:

  • The developed organ-on-chip platform successfully creates vascularized human cardiac microtissues.
  • This model facilitates studies on endothelial cell barrier function and responses to stimuli.
  • The platform is suitable for investigating drug effects and inflammatory processes in cardiac tissue.