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Expression, Solubilization, and Purification of Eukaryotic Borate Transporters
Published on: March 7, 2019
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SVCT2/SLC23A2 is a sodium-dependent urate transporter: functional properties and practical application
Yu Toyoda1, Hiroshi Miyata1, Ryuichiro Shigesawa1
1Department of Pharmacy, The University of Tokyo Hospital, Bunkyo-ku, Tokyo, Japan.
The Journal of Biological Chemistry
|June 30, 2023
Summary
Sodium-dependent vitamin C transporter 2 (SVCT2) is identified as a novel urate transporter. This discovery enhances our understanding of urate handling and may reveal new targets for related diseases.
Area of Science:
- Biochemistry
- Molecular Biology
- Physiology
Background:
- Existing urate transporters do not fully explain urate handling, indicating undiscovered mechanisms.
- SLC2A12, a known urate transporter, also exports ascorbate (vitamin C) and cooperates with the importer SVCT2.
- The dual role of SLC2A12 and its interaction with SVCT2 suggest SVCT2 might also transport urate.
Purpose of the Study:
- To investigate the hypothesis that SVCT2 possesses urate transport activity.
- To characterize the interaction between SVCT2, urate, and vitamin C.
- To develop novel assays for identifying urate transporters and variants.
Main Methods:
- Cell-based analyses were performed using mammalian cells engineered to express SVCT2.
- SVCT2-mediated urate transport was assessed, including inhibition by vitamin C.
- Experiments were replicated using mouse Svct2 to confirm findings.
Main Results:
- SVCT2 was confirmed as a novel urate transporter in mammalian cells.
- Vitamin C inhibited SVCT2-mediated urate transport, with a half-maximal inhibitory concentration of 36.59 μM.
- Similar urate transport activity was observed for mouse Svct2.
Conclusions:
- SVCT2 functions as a sodium-dependent urate importer, expanding the known urate transport machinery.
- The sensitivity of SVCT2 urate transport to vitamin C suggests physiological relevance.
- A new cell-based urate efflux assay utilizing SVCT2 was established for future research.
Keywords:
ABCG2anion transportascorbateascorbic acidcell-based urate efflux assaypurineurate importeruric acidvitamin C transporterMore Related Videos
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