Multinuclear 1D and 2D NMR with ¹⁹F-Photo-CIDNP hyperpolarization in a microfluidic chip with untuned microcoil

Area of Science:

• Analytical Chemistry
• Spectroscopy
• Materials Science

Background:

• Nuclear Magnetic Resonance (NMR) spectroscopy is a powerful tool for molecular analysis.
• Current limitations include low sensitivity and complex, expensive hardware.
• Wider adoption of NMR is hindered by these challenges.

Purpose of the Study:

• To develop a highly sensitive and cost-effective NMR system.
• To address the limitations of poor sensitivity and hardware complexity in NMR.
• To enable advanced NMR experiments on a microfluidic chip.

Main Methods:

• Utilized a microfluidic NMR chip with a single planar-spiral microcoil.
• Integrated photochemically induced dynamic nuclear polarization (photo-CIDNP) for sensitivity enhancement.
• Employed an untuned circuit for broadband and multi-nuclide capabilities.

Main Results:

• Achieved orders of magnitude sensitivity enhancement via photo-CIDNP.
• Enabled rapid detection of samples in the lower picomole range (nLOD_f,600 of 0.01 nmol Hz^1/2).
• Demonstrated capability for advanced hetero-, di-, and trinuclear 1D and 2D NMR experiments.

Conclusions:

• The developed NMR chip significantly enhances sensitivity and reduces hardware complexity and cost.
• This technology overcomes major limitations, paving the way for broader NMR applications.
• Performance is comparable to state-of-the-art NMR instruments.