In Vitro Antibacterial Activity of Honey against Gram Negative Bacterial Isolates

Topical application of honey results in disinfecting the wound infection promptly and also expedites healing. Being cheap and widely available, honey can be an excellent alternative to be used as a topical antimicrobial agent. This study observes the in vitro growth inhibitory activity of different concentration of honey for different bacterial strains. This experimental study had been carried out in the Department of Pharmacology and Therapeutics of Sir Salimullah Medical College and Mitford Hospital (SSMC), Dhaka, Bangladesh in collaboration with Microbiology, Department of Sir Salimullah Medical College and Mitford Hospital (SSMC), Dhaka, Bangldesh over a period of one year from July 2018 to June 2019. Using the Agar dilution method to detect the antimicrobial activity of honey on 18 bacterial isolates belong to enterobacteriaceae family namely, 8 bacterial isolates of Salmonella Enterica Serovar Typhi, Five (5) bacterial isolates of Escherichia coli and 5 bacterial isolates of Pseudomonas aeruginosa. The mean of the minimum inhibitory concentration (MIC) of honey for the isolates of Salmonella enterica serovar typhi was 15.35±12.39mg/ml, ranged between 3.56 and 41.6mg/ml (0.25% - 3.0% v/v). In case of the isolates of Escherichia coli the mean MIC of honey recorded at 28.53±16.18mg/ml and the growth was ranged between 7.10 and 48.3mg/ml (0.5% - 3.50% v/v). And the mean of the MIC of honey for the isolates of Pseudomonas aeruginosa was 20.31±13.20mg/ml, ranged between 10.63 and 41.6mg/ml (0.75% - 3.0% v/v). The brilliant antibacterial activity of honey against clinical bacterial isolates indicates the usefulness of honey in clinical practice against bacterial infection.