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Updated: Jul 24, 2025

Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins
Published on: April 27, 2019
Robust production of active Ulp1 (SUMO protease) from inclusion bodies
Bala Kota Reddy Patakottu1, Vikram Reddy Vedire1, Chereddy Ramamohan Reddy1
1LOKA Biosciences Pvt Ltd, ALEAP Industrial Estate, Road No.11, PLOT 14 & 15, Pragati Nagar, Hyderabad, 500090, India.
Abstract:
High yield purification of Ulp1 is required during the isolation and purification of SUMO-tagged recombinant proteins. However, when expressed as a soluble protein, Ulp1 is toxic to E. coli host cells and most of the protein forms inclusion bodies. The extraction of insoluble Ulp1 followed by its purification and refolding into its active form is a lengthy and costly procedure. In our present study, we developed a simple, cost effective procedure for the large scale production of active Ulp1 that can be used for industrial scale requirements.
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