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An investigation of a collection of yellow-pigmented Pseudomonas
Abstract:
Thirty-six strains of yellow-pigmented Pseudomonas from clinical as well as non-clinical material and 11 reference strains of Pseudomonas were investigated by means of conventional bacteriological methods (a total of 53 different tests). Eighteen of the 36 yellow-pigmented strains could be classified as P. paucimobilis. Apart from the presence of lipid inclusions on beta-hydroxybutyrate, hydrolysis of DNA, and Tween 80 our results showed a high degree of accordance with other investigations. Eight strains showed characteristics compatible with inclusion in the CDC VE group; one orange-yellow strain showed the characteristics of P. vesicularis, and one was a pyoverdin negative, yellow P. putida. Eight strains remained unidentified. Strains of P. paucimobilis were most often resistant to antibiotics used for P. aeruginosa infections (viz. piperacillin, cefsulodin, ceftazidime) while the strains of the CDC VE group were often susceptible. Most strains were susceptible to the aminoglycosides. The difficulties in distinguishing yellow-pigmented strains of Pseudomonas from Flavobacterium spp. or Xanthomonas spp. are discussed.
Insights
This study classified yellow-pigmented Pseudomonas strains, identifying most as Pseudomonas paucimobilis. These strains often showed antibiotic resistance, unlike the CDC VE group, highlighting challenges in differentiating Pseudomonas species.
Area of Science:
- Microbiology
- Bacteriology
- Clinical Diagnostics
Background:
- Yellow-pigmented Pseudomonas species present diagnostic challenges.
- Accurate identification is crucial for clinical and environmental microbiology.
Purpose of the Study:
- To classify and characterize yellow-pigmented Pseudomonas strains.
- To assess antibiotic susceptibility patterns of identified strains.
Main Methods:
- Conventional bacteriological methods and 53 different tests were employed.
- Analysis included lipid inclusions, DNA hydrolysis, and Tween 80 hydrolysis.
- Antibiotic susceptibility testing was performed.
Main Results:
- Eighteen of 36 yellow-pigmented strains were identified as Pseudomonas paucimobilis.
- Eight strains fit the CDC VE group criteria, one was Pseudomonas vesicularis, and one was Pseudomonas putida.
- Pseudomonas paucimobilis strains exhibited resistance to common Pseudomonas aeruginosa antibiotics, while CDC VE group strains were often susceptible.
Conclusions:
- Accurate differentiation of yellow-pigmented Pseudomonas from related genera like Flavobacterium and Xanthomonas is difficult.
- Antibiotic resistance patterns can aid in distinguishing between Pseudomonas paucimobilis and CDC VE group strains.