Related Experiment Video

Updated: Jul 24, 2025

Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria
11:32

Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria

Published on: February 23, 2014

15.1K

Metagenomic-based pathogen surveillance for children with severe pneumonia in pediatric intensive care unit

Meijin Li1, Jing Wang2, Zhongwei Yao1

  • 1Department of Pediatric Intensive Care Unit, Guangdong Women and Children Hospital, Guangzhou, China.

Frontiers in Public Health
|July 3, 2023
PubMed
Abstract

Insights

Metagenomic next-generation sequencing (mNGS) identified 80 pathogens in children with severe pneumonia. Respiratory syncytial virus (RSV) and rhinovirus were common, with high co-infection rates observed.

Area of Science:

  • Pediatric Infectious Diseases
  • Microbial Genomics
  • Diagnostic Microbiology

Background:

  • Pneumonia is a major cause of childhood illness and death.
  • Metagenomic next-generation sequencing (mNGS) offers a comprehensive approach to identifying pathogens in severe lung infections.

Purpose of the Study:

  • To investigate the microbial landscape of severe pneumonia in pediatric patients.
  • To compare the efficacy of mNGS with conventional diagnostic methods.

Main Methods:

  • Bronchoalveolar lavage fluid (BALF) samples were collected from 262 children with suspected pneumonia.
  • Samples were analyzed using both mNGS and conventional microbiological tests.

Main Results:

  • 80 pathogens were identified in total.
  • Respiratory syncytial virus (RSV), Staphylococcus aureus, and rhinovirus were the most frequent.
  • Co-infections were prevalent (58.96%), with bacterial-viral coinfections being most common.
  • Age-specific pathogen prevalence was noted, including Pneumocystis jirovecii in infants under 6 months.

Conclusions:

  • mNGS is crucial for understanding the epidemiology of pediatric pneumonia.
  • Advanced diagnostics like mNGS enhance pathogen detection and inform clinical management.
Keywords:
PICUco-infectionmNGSpathogenssevere pneumonia

More Related Videos

Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins06:05

Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins

Published on: September 30, 2014

13.6K
Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip06:11

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Published on: March 29, 2024

1.9K

Related Concept Videos

Pneumonia III: Complications and Assessment01:30

Pneumonia III: Complications and Assessment

Pneumonia poses the potential for numerous complications that warrant consideration. These complications include the following:
275
Principles of Disease Surveillance01:26

Principles of Disease Surveillance

Disease surveillance is the systematic collection, analysis, and interpretation of health data essential to the planning, implementation, and evaluation of public health practice. This process integrates data dissemination to entities responsible for preventing and controlling disease, injury, and disability. Surveillance systems provide crucial information for action, helping public health authorities make informed decisions to manage and prevent outbreaks, ensure public safety, optimize...
127
Pneumonia I: Introduction01:30

Pneumonia I: Introduction

Pneumonia is an acute respiratory infection that targets the lungs, specifically the alveoli. These tiny air sacs, essential for oxygen exchange, become engorged with pus and fluid, severely hindering breathing, decreasing oxygen absorption, and causing significant pain and discomfort during respiration.
Risk Factors
Various factors influence the likelihood of developing pneumonia. Age plays a crucial role, with infants, children under two, and individuals over 65 at increased risk due to their...
281
Pneumonia II: Pathophysiology01:29

Pneumonia II: Pathophysiology

The pathophysiology of pneumonia involves the following steps:
359
Pneumonia V: Nursing management and Prevention01:30

Pneumonia V: Nursing management and Prevention

Nursing management of pneumonia involves promoting airway patency, facilitating rest and conserving energy, encouraging fluid intake, maintaining nutrition, and educating patients.
The nurse must practice strict medical asepsis and adhere to infection control guidelines to minimize healthcare-associated infections.
Enhance airway patency
Position the patient correctly to facilitate drainage of the affected lung segments. Manual or mechanical percussion and vibration can also be employed....
2.2K
Pneumonia IV: Management01:28

Pneumonia IV: Management

The treatment of pneumonia varies based on its severity and the causative pathogen. Here is a structured approach to managing pneumonia, integrating pharmaceutical and supportive care strategies.
Bacterial Pneumonia Treatment
For bacterial pneumonia, antibiotics serve as the cornerstone of therapy. Initial treatment often begins with empirical antibiotics, tailored to the anticipated causative organism and adjusted based on culture results. Key antibiotic choices include:
367