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Updated: Jul 24, 2025

Author Spotlight: Universal Molecular Retention with 11-Fold Expansion Microscopy
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Ten-fold Robust Expansion Microscopy.

Hugo G J Damstra1, Boaz Mohar2, Mark Eddison2

  • 1Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands.

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|July 3, 2023
PubMed
Summary
This summary is machine-generated.

Ten-fold Robust Expansion Microscopy (TREx) is a new method that expands biological samples tenfold for high-resolution imaging. This technique requires no specialized equipment and works on tissues and cells, revealing ultrastructural details.

Keywords:
Antibody labelingExpansion microscopyLight microscopyProtein stainSuper resolutionUltrastructure

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Area of Science:

  • Biotechnology
  • Microscopy
  • Cell Biology

Background:

  • Light microscopy faces diffraction limits, hindering subcellular resolution.
  • Expansion Microscopy (ExM) overcomes these limits by physically expanding samples.
  • Existing ExM methods can be complex or require specialized equipment.

Purpose of the Study:

  • To develop a novel, robust, and user-friendly Expansion Microscopy (ExM) method.
  • To achieve tenfold isotropic expansion for high-resolution imaging of biological samples.
  • To enable ultrastructural context for subcellular localization studies.

Main Methods:

  • Systematic exploration of the ExM recipe space.
  • Development of a novel method termed Ten-fold Robust Expansion Microscopy (TREx).
  • Application of TREx to thick mouse brain tissue sections and cultured human cells.

Main Results:

  • TREx achieves a consistent tenfold physical expansion of samples.
  • The method requires no specialized equipment or complex procedures.
  • TREx enables high-resolution subcellular imaging in a single expansion step.
  • Combined TREx with small molecule stains for total protein and membranes to provide ultrastructural context.

Conclusions:

  • TREx is a robust and accessible method for achieving high-resolution microscopy.
  • This technique significantly enhances the ability to visualize subcellular structures and protein localization.
  • TREx offers a powerful tool for biological research without specialized infrastructure.