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Updated: Jul 24, 2025

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
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Enterovirus D68 capsid formation and stability requires acidic compartments

Ganna Galitska1, Alagie Jassey1, Michael A Wagner1

  • 1Department of Microbiology and Immunology, University of Maryland School of Medicine, 685 W. Baltimore St, Baltimore, MD 21201, USA.

Biorxiv : the Preprint Server for Biology
|July 3, 2023
PubMed

Insights

Enterovirus D68 requires specific organelle acidification for capsid formation. Inhibiting this process disrupts viral replication and causes cellular changes, unlike poliovirus.

Area of Science:

  • Virology
  • Cell Biology

Background:

  • Enterovirus D68 (EV-D68) is a picornavirus linked to acute flaccid myelitis (AFM).
  • Understanding EV-D68 pathogenesis is limited, often relying on poliovirus models.
  • Low pH promotes poliovirus capsid maturation, but EV-D68's requirements are less understood.

Approach:

  • Investigated the role of compartment acidification in EV-D68 infection.
  • Examined cellular and viral phenotypes upon inhibition of acidification.
  • Identified a critical time window for acidification's role in EV-D68 lifecycle.

Key Points:

  • Inhibiting compartment acidification during a specific window (3-4hpi) impairs EV-D68 capsid formation and maintenance.
  • This leads to altered viral replication organelles clustering.
  • Acidification is crucial during the transition from RNA replication to virion assembly.

Conclusions:

  • EV-D68 has distinct acidification requirements compared to poliovirus.
  • Compartment acidification is a critical, time-sensitive step in EV-D68 virion production.
  • Findings reveal novel insights into EV-D68 replication dynamics and potential therapeutic targets.
Keywords:
autophagosomesautophagycellular acidificationenterovirus D68multi-vesicular bodies (MVB)picornavirusespoliovirusreplication organelles (ROs)virion assemblyvirus maturation“transition point”

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