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Updated: Jul 24, 2025
09:08
Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
Published on: July 27, 2021
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Enterovirus D68 capsid formation and stability requires acidic compartments
Ganna Galitska1, Alagie Jassey1, Michael A Wagner1
1Department of Microbiology and Immunology, University of Maryland School of Medicine, 685 W. Baltimore St, Baltimore, MD 21201, USA.
Biorxiv : the Preprint Server for Biology
|July 3, 2023
Summary
Enterovirus D68 requires specific organelle acidification for capsid formation. Inhibiting this process disrupts viral replication and causes cellular changes, unlike poliovirus.
Area of Science:
- Virology
- Cell Biology
Background:
- Enterovirus D68 (EV-D68) is a picornavirus linked to acute flaccid myelitis (AFM).
- Understanding EV-D68 pathogenesis is limited, often relying on poliovirus models.
- Low pH promotes poliovirus capsid maturation, but EV-D68's requirements are less understood.
Approach:
- Investigated the role of compartment acidification in EV-D68 infection.
- Examined cellular and viral phenotypes upon inhibition of acidification.
- Identified a critical time window for acidification's role in EV-D68 lifecycle.
Key Points:
- Inhibiting compartment acidification during a specific window (3-4hpi) impairs EV-D68 capsid formation and maintenance.
- This leads to altered viral replication organelles clustering.
- Acidification is crucial during the transition from RNA replication to virion assembly.
Conclusions:
- EV-D68 has distinct acidification requirements compared to poliovirus.
- Compartment acidification is a critical, time-sensitive step in EV-D68 virion production.
- Findings reveal novel insights into EV-D68 replication dynamics and potential therapeutic targets.
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